Product Name

FKBP51 Antibody

Catalog #

SMC-138C

Package size

25ug

Price

$139.00 USD

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Alternate Product Sizes

SMC-138D

Type

Monoclonal

Conjugate

N/A

Datasheet

SMC 138 Heat Shock Protein 90 Co Chaperone, FKBP51

Description

Anti-FKBP51

Research Area

Heat Shock

Alternative Names

AIG6, FK506 binding protein 5, FKBP5, FKBP54, Hsp90 binding immunophilin, p54, Pplase, Ptg10, Rotamase, T cekk FK506 binding protein antibody

Clone Number

Hi51B

Host Species

Mouse

Isotype

IgG

Immunogen

Synthetic peptide corresponding to the residues of human FKBP51

Applications

WB, IF

Species Reactivity

Canine, Hamster, Human, Mouse, Rat, Rabbit

Accession Number

NP_001139247.1

Gene ID

2289

SwissProt

Q13451

Background Info

Detects an ~51kDa protein representing FKBP51 in cell lysate. Also detects FKBP51 in whole tissue extracts from rat kidney and rat and mouse testes.

Recommended Dilutions

1:2000 (WB)

Form

Protein G Purified

Storage Buffer

PBS, 50% glycerol, 0.09% sodium azide

Concentration

1mg/mL

Certificate of Analysis

A 1:2000 dilution was sufficient for detection of FKBP51 in ~50µg total protein using WB anaylsis.

Storage Temp

-20°C

Shipping Temp

Blue Ice or 4°C

IF localization of FKBP51 antibody in normal MK cells (shown in red). 
Courtesy of the Hospital Henri Mondor, France.


Western blot analysis of FKBP51 in HeLa cell lysates, using a 1:1000 dilution of SMC-138.

Research Background

Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90 (4). Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).

References

1. Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F.(2003) J. Biol. Chem. 278(19): 17388-17394.
2. Davies T.H., Ning Y.N., and Sanchez E.R. (2002) J Biol. Chem. 277 (7): 4597-4600.
3. Wu B. et al. (2004) Proc. Natl. Acad. Sci. USA. 101(22): 8348-8353.
4. Denny W.B., Prapapanich V., Smith D.F., and Scammell J.G. (2005) Endocrinology 146(7): 3194-3201.
5. Hubler T.R. et al. (2003) Endocrinology 144(6): 2380- 2387.