Product Name

CENP-A Antibody

Catalog #

SMC-202D

Package size

100ug

Price

$265.00 USD

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Type

Monoclonal

Datasheet

SMC 202 CENP A Cell Signaling

Description

Anti-CENP-A

Research Area

Cell Signaling

Alternative Names

CENP A, cenpa, Centromere autoantigen A, Histone H3 like centromeric protein A

Clone Number

5A7-2E11

Host Species

Mouse

Isotype

IgG1

Immunogen

Synthetic peptide corresponding to a portion of human CENP-A.

Applications

WB

Species Reactivity

Human. Other species not yet tested.

Accession Number

NP_001035891.1

Gene ID

1058

SwissProt

P49450

Background Info

Recognizes human CENP-A. Detects bands of ~18kDa.

Recommended Dilutions

1:1000 (WB)

Form

Protein G Purified

Storage Buffer

PBS pH7.2, 50% glycerol, 0.09% sodium azide

Concentration

1mg/mL

Certificate of Analysis

1 μg/mL of SMC-202 was sufficient for detection of CENPA in 20μg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Storage Temp

-20°C

Shipping Temp

Blue Ice or 4°C

This antibody is available conjugated to several dyes. Please select one of the conjugates from the lists below:

Western blot analysis of CENP-A in U2OS lysates using a 1:1000 dilution of SMC-202.

Research Background

A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain.
CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized
centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.

References

1. Rieder C.L., et al. (1998) Trends Cell Biol. 8: 310-318.
2. Choo K.H. (2000) Trends Cell Biol. 10: 182-188.
3. Muro Y., et al. (2000) Clin. Exp. Immunol. 120: 218-223.
4. Howman E.V., et al. (2000) Proc. Natl. Acad. Sci. USA 97: 1148-1153.