Antibodies - Heat Shock Proteins, Oxydative Stress, Apoptosis, Cell Signaling

Home > Products > Antibodies > SPC-108A

Product Name: Calnexin-CT Antibody
Catalog #: SPC-108A
Package size: 50ul
Price: $144.00 USD

Add to Cart

Bulk Quote
Alternate Product Sizes: SPC-108B
Type: Polyclonal
Conjugate: N/A
Datasheet: SPC 108 Calnexin CT Cell Signaling
Description: Anti-Calnexin (CT)
Research Area: Cell Signaling, Organelle Markers
Alternative Names: CANX, CNX, IP90, P90
Clone Number: N/A
Host Species: Rabbit
Isotype: N/A
Immunogen: Dog calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Applications: WB, IHC, ICC, IP, Flow cytometry, IF
Species Reactivity: Human, Monkey, Mouse, Rat, Bovine, Chicken (weak), Dog, Guinea pig, Hamster, Pig, Quail, Rabbit, Sheep, Drosophila (weak), Xenopus (weak)
Accession Number: NP_001003232.1
Gene ID: 403908
SwissProt: P24643
Background Info: Detects ~90kDa
Recommended Dilutions: 1:2000 (WB)
Form: Rabbit antiserum
Storage Buffer: Rabbit antiserum
Concentration: N/A
Certificate of Analysis: A 1:1000 dilution of SPC-108 was sufficient for detection of Calnexin in 10μg of HeLa cell lysate by ECL immunoblot analysis.
Storage Temp: -20°C
Shipping Temp: Blue Ice or 4°C

IF analysis of Calnexin-CT using SPC-108, tested in HeLa cell lysates.

Western blot analysis of Calnexin-CT in rat tissues using a 1:1000 dilution of SPC-108.

Research Background: Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulitsubunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
References: 1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90.
2. Tjoelker L.W., et al. (1994) Biochemistry 33: 3229.
3. Schrag J. et al. (2001) Molecular Cell 8(3): 633-644.
4. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813- 40819.
5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460.
6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97- 103.
7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.

Full Name:	*
Company:	*
Phone:	*
Email:	*
Address:	*
Address 2:
City:	*
State/Province:
Country:	*
Zip/Postal:	*
Primary Work Interest:	*
Send Print Catalog?	* Yes No
Newsletter Sign-Up:	Cellular Stress Oxidative Stress Ion Channels & Transporters Apoptosis
Check box before sending form:	*