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Product Name: Calnexin-CT Antibody
Catalog #: SPC-182A
Package size: 50ug
Price: $149.00 USD

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Alternate Product Sizes: SPC-182B
Type: Polyclonal
Conjugate: N/A
Datasheet: SPC 182 Calnexin CT Cell Signaling
Description: Anti-Calnexin (CT)
Research Area: Cell Signaling, Organelle Markers
Alternative Names: CANX, CNX, IP90, P90
Clone Number: N/A
Host Species: Rabbit
Isotype: N/A
Immunogen: Dog calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Applications: WB, IP, ICC, IHC, Flow Cytometry
Species Reactivity: Human, Monkey, Mouse, Rat, Bovine, Chicken (weak), Dog, Guinea pig, Hamster, Pig, Quail, Rabbit, Sheep, Drosophila (weak), Xenopus (weak)
Accession Number: NP_001003232.1
Gene ID: 403908
SwissProt: P24643
Background Info: Detects ~90kDa
Recommended Dilutions: 1:2000 (WB)
Form: Protein A Purified
Storage Buffer: PBS pH7.2, 50% glycerol, 0.09% sodium azide, 0.09% sodium azide
Concentration: 1mg/mL
Certificate of Analysis: A 1:2000 dilution of SPC-182 was sufficient for detection of Calnexin in 10μg of HeLa cell lysate by ECL immunoblot analysis.
Storage Temp: -20°C
Shipping Temp: Blue Ice or 4°C

Western blot analysis of purified calnexin-CT in a rat tissue mix using a 1:1000 dilution of SPC-182.

Research Background: Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulitsubunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
References: 1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90.
2. Tjoelker L.W., et al. (1994) Biochemistry 33: 3229.
3. Schrag J. et al. (2001) Molecular Cell 8(3): 633-644.
4. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813- 40819.
5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460.
6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97- 103.
7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.

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