Handling Instructions | Amyloid Beta Proteins
| Product Name | Amyloid Beta Proteins (Monomers, Oligomers, Pre-formed Fibrils (PFFs)) |
| Storage Conditions | Monomers, Oligomers, and unsonicated Pre-formed Fibrils (PFFs): -80ºC; 6 months+ |
| Freeze/Thaw Cycles | 1 additional freeze/thaw cycle maximum |
| Thawing Conditions | Monomer film and Pre-formed Fibrils (PFFs): Room temperature
Monomer re-suspension and Oligomers: 4ºC or on ice |
| Storage (Thawed Protein) | Monomers: Amyloid beta monomers are shipped as a dried peptide film. They must be re-suspended prior to use. Re-suspensions should be used immediately to avoid aggregation. If re-frozen at -80ºC, re-suspensions should be spundown ≥14,000 xg at 4ºC for ≥5 minutes to remove any aggregate formed during freeze/thaw cycle.
Oligomers: 4oC or on ice. Use immediately. Pre-formed Fibrils (PFFs): Room temperature. Do not store Pre-formed Fibrils (PFFs) on ice or at 4ºC. |
| Mixing/Pipetting | Amyloid beta pre-formed fibrils (PFFs) and oligomers are in solution, and should be pipetted up and down immediately before use to ensure homogeneity.
Amyloid beta monomer preps are in a dried peptide film. Once re-suspended, monomers are in solution and should be pipetted up and down to ensure homogeneity. |
| Sonication |
For best results, Pre-formed Fibrils (PFFs) should be sonicated immediately prior to use. Mixing/pipetting is also needed before and after sonication to ensure homogeneity.
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| Safety | Appropriate laboratory attire: Gloves, face mask (VWR, 414004-670), and protective goggles should be used for all work involving fibrils, especially where aerosols may be created (for example during probe sonication). Clean any spills with a solution of 10% bleach or 1M NaOH. |