Handling Instructions | SOD & TTR Proteins
| Product Name | SOD and TTR (Monomers, Pre-formed Fibrils, Filaments) |
| Storage Conditions | Monomers, and unsonicated Pre-formed Fibrils or Filaments: -80ºC; 1 year+
Sonicated Pre-formed Fibrils: -80ºC; 8 weeks |
| Freeze/Thaw Cycles | 2 freeze/thaw cycles maximum |
| Thawing Conditions | Pre-formed Fibrils and Filaments: 37ºC
Monomers: on ice or at 4ºC |
| Storage (Thawed Protein) | Pre-formed Fibrils and Filaments: Room temperature. Do not store Pre-formed Fibrils or filaments on ice or at 4ºC; Pre-formed Fibrils and filaments are unstable at 4ºC and will degrade rapidly. Pre-formed Fibrils and filaments are stable at room temperature and can be kept on the bench when setting up experiments.
Monomers: On ice or at 4ºC. Monomers may start to aggregate if left at room temperature or 37ºC for prolonged amounts of time. |
| Mixing/Pipetting | Pre-formed Fibrils are in suspension and should be vortexed briefly and pipetted up and down immediately before use to ensure homogeneity.
Monomer preps are in solution and should be pipetted up and down after thawing to ensure homogeneity. Filaments are a mixed population of soluble and insoluble material and should be vortexed briefly and pipetted up and down immediately before use to ensure homogeneity. |
| Sonication |
For best results, Pre-formed Fibrils and filaments should be sonicated immediately prior to use. Mixing/pipetting is also needed before and after sonication to ensure homogeneity.
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| Safety | Appropriate laboratory attire: gloves, face mask (VWR, 414004-670), and protective goggles for all work involving fibrils, especially where aerosols may be created (for example during probe sonication). Clean any spills with a solution of 10% bleach or 10% SDS in water. |