Handling Instructions | Synuclein Proteins

Product Name	Alpha Synuclein, Beta Synuclein, Gamma Synuclein (Monomers, Oligomers, Pre-formed Fibrils (PFFs), and Filaments)
Storage Conditions	Monomers, Oligomers, and unsonicated Pre-formed Fibrils or Filaments: -80ºC; 1 year+ Sonicated Pre-formed Fibrils: -80ºC; 8 weeks
Freeze/Thaw Cycles	2 freeze/thaw cycles maximum
Thawing Conditions	Pre-formed Fibrils, Oligomers, and Filaments: 37ºC Monomers: on ice or at 4ºC
Storage (Thawed Protein)	Pre-formed Fibrils, Oligomers, and Filaments: Room temperature. Do not store Pre-formed Fibrils or filaments on ice or at 4ºC; Pre-formed Fibrils and filaments are unstable at 4ºC and will degrade rapidly. Pre-formed Fibrils, oligomers, and filaments are stable at room temperature and can be kept on the bench when setting up experiments. Monomers: On ice or at 4ºC. Monomers may start to aggregate if left at room temperature or 37ºC for prolonged amounts of time.
Mixing/Pipetting	Pre-formed Fibrils are in suspension and should be vortexed briefly and pipetted up and down immediately before use to ensure homogeneity. Monomer and oligomer preps are in solution and should be pipetted up and down after thawing to ensure homogeneity. Filaments are a mixed population of soluble and insoluble material and should be vortexed briefly and pipetted up and down immediately before use to ensure homogeneity.
Sonication	For best results, Pre-formed Fibrils and filaments should be sonicated immediately prior to use. Mixing/pipetting is also needed before and after sonication to ensure homogeneity. No sonication is necessary for monomers and oligomers.
Safety	Appropriate laboratory attire: gloves, face mask (VWR, 414004-670), and protective goggles for all work involving fibrils, especially where aerosols may be created (for example during probe sonication). Clean any spills with a solution of 10% bleach or 10% SDS in water.