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Anti-ATG2A Antibody
| Product Name | ATG2A Antibody |
| Description |
Rabbit Anti-Human ATG2A Polyclonal |
| Species Reactivity | Human, Mouse |
| Applications | WB, ICC/IF |
| Antibody Dilution | WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
| Host Species | Rabbit |
| Immunogen Species | Human |
| Immunogen | Synthetic peptide from the C-terminal of human ATG2A |
| Concentration | 1 mg/ml |
| Conjugates |
APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated |
| Field of Use | Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only. |
Properties
| Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated |
| Storage Temperature | -20ºC, Conjugated antibodies should be stored according to the product label |
| Shipping Temperature | Blue Ice or 4ºC |
| Purification | Peptide Affinity Purified |
| Clonality | Polyclonal |
| Specificity | Predicted molecular weights at ~213kDa. Other observed bands around ~90-100kDa to be expected. |
| Cite This Product | StressMarq Biosciences Cat# SPC-652, RRID: AB_2705393 |
| Certificate of Analysis | A 1:1000 dilution of SPC-652 was sufficient for detection of ATG2A on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody. |
Biological Description
| Alternative Names | ATG2 autophagy related homolog A Antibody, ATG2a_HUMAN Antibody, BC023754 Antibody, KIAA0404 Antibody, 1810013C15Rik Antibody |
| Research Areas | Autophagy, Cancer, Cardiovascular System, Heart, Neuroscience |
| Cellular Localization | Peripheral membrane protein, Preautophagosomal Structure Membrane |
| Accession Number | NP_055919.2 |
| Gene ID | 23130 |
| Swiss Prot | Q2TAZ0 |
| Scientific Background | ATG2A belongs to the ATG2 family. It is required for both autophagosome formation and regulation of lipid droplet morphology and dispersion (1). |
| References | 1. Velikkakath A.K., Nishimura T., Oita E., Ishihara N., Mizushima N. (2012) Mol Biol Cell. 23(5): 896-909. |
Product Images
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG2A Antibody (D) Composite.
Western blot analysis of Mouse Brain showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa.
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa.
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