Anti-HSP110 Antibody

Rabbit Anti-Hamster HSP110 Polyclonal

Catalog No. SPC-195

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Species Reactivity Hu, Ms, Rt, Bv, Mk, Hm, Sh, Ys, Shk
Applications WB IHC ICC/IF FCM IP
SKU: SPC-195 Categories: ,

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SPC-195_HSP110_Antibody_IHC_Mouse_backskin_1.png
Rabbit Anti-HSP110 Antibody used in Western blot (WB) on Cell line lysates (SPC-195)
Product Name HSP110 Antibody
Description

Rabbit Anti-Hamster HSP110 Polyclonal

Species Reactivity Bovine, Gummy Shark (Mustelus antarcticus), Hamster, Human, Monkey, Mouse, Rat, School Shark (Galeorhinus galeus), Shark, Sheep, Yeast
Applications WB, IHC, ICC/IF
Antibody Dilution WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Hamster
Immunogen Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse
Concentration 1 mg/ml
Conjugates APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20ºC, Conjugated antibodies should be stored according to the product label
Shipping Temperature Blue Ice or 4ºC
Purification Protein A purified
Clonality Polyclonal
Specificity Detects ~110kDa.
Cite This Product StressMarq Biosciences Cat# SPC-195, RRID: AB_2119373
Certificate of Analysis 1 µg/ml of SPC-195 was sufficient for detection of HSP110 in 10 µg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names Heat shock 105kDa alpha Antibody, heat shock 105kDa beta Antibody, HSP105 Antibody, HSP105A Antibody, HSP105B Antibody, HSP110 Antibody, HSPH1 Antibody, NY CO 25 Antibody
Research Areas Cancer, Cell Signaling, Chaperone Proteins, Heat Shock, Protein Trafficking
Cellular Localization Cytoplasm
Accession Number NP_006635.2, NP_006635.3
Gene ID 10808
Swiss Prot Q92598, Q92599
Scientific Background HSP110 belongs to a family of large stress proteins known as the HSP110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied HSP70 family. They share 30-33% amino acid identity, mostly in the conserved ATP-binding domain (1). HSP110 cooperates with HSP70 in protein folding in the eukaryotic cytosol (2). In mammals, HSP110 is constitutively expressed, but exhibits particularly high levels in the brain. Both HSP70 and HSP110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of HSP110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of HSP70 (3). Studies also suggest that HSP110 can be used in heat shock protein-based cancer immunotherapy (4).
References 1. Lee-Yoon D., et al. (1995) J Biol Chem. 270: 15725-15733.
2. Dragovic Z., et al. (2006) EMBO J. 25: 2519-2528.
3. Nakamura J., et al. (2008) Stroke. 39(10): 2853-2859.
4. Subject D.R. (2001) Health Research Inc. Report number AS90893: 1-84.

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative Solution. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Positive epidermal, dermal and muscle staining.</p>

Immunohistochemistry analysis using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative Solution. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Positive epidermal, dermal and muscle staining.

<p>Western blot analysis of Human Cell line lysates showing detection of HSP110 protein using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.</p>

Western blot analysis of Human Cell line lysates showing detection of HSP110 protein using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

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