Anti-Cav3.2 Antibody [N55/10 (Formerly sold as S55-10)]

Mouse Anti-Human Cav3.2 Monoclonal IgG1

Catalog No. SMC-303

5 out of 5 based on 1 customer rating
Species Reactivity Hu, Ms, Rt
Applications WB IHC ICC/IF FCM IP
SKU: SMC-303D Categories: ,

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SMC-303_Cav32_Antibody_N55-10_ICC-IF_Human_Neuroblastoma-cells-SH-SY5Y-Composite-1.png
Mouse Anti-CaV3.2 Calcium Channel Antibody [N55/10] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-303)Mouse Anti-CaV3.2 Calcium channel antibody [N55/10] used in Immunohistochemistry (IHC) on Human frozen brain section (SMC-303)Mouse Anti-Cav3.2 Antibody [N55/10] used in Western Blot (WB) on Rat brain membrane lysate (native) (SMC-303)
Product Name Cav3.2 Antibody
Description

Mouse Anti-Human Cav3.2 Monoclonal IgG1

Species Reactivity Human, Mouse, Rat
Applications WB, IHC, ICC/IF, IP, AM
Antibody Dilution WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Human
Immunogen Fusion protein amino acids 1019-1293 (II-III loop) of human Cav3.2
Concentration 1 mg/ml
Conjugates APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20ºC, Conjugated antibodies should be stored according to the product label
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number N55/10 (Formerly sold as S55-10)
Isotype IgG1
Specificity Detects ~260kDa. No cross-reactivity against Cav1.3.
Cite This Product StressMarq Biosciences Cat# SMC-303, RRID: AB_2069540
Certificate of Analysis 1 µg/ml of SMC-303 was sufficient for detection of Cav3.2 in 10 µg of HEK cell lysate expressing Cav3.2 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names Cav3.2 Antibody, CACNA1H Antibody, CACNA1HB Antibody, calcium channel Antibody, voltage-dependent Antibody, T type Antibody, alpha 1H subunit Antibody, calcium channel Antibody, voltage-dependent Antibody, T type Antibody, alpha 1Hb subunit Antibody, ECA6 Antibody, EIG6 Antibody, FLJ90484 Antibody, Low-voltage-activated calcium channel alpha1 3.2 subunit Antibody, low-voltage-activated calcium channel alpha13.2 subunit Antibody, voltage dependent t-type calcium channel alpha-1H subunit Antibody, voltage-dependent T-type calcium channel subunit alpha-1H Antibody, voltage-gated calcium channel alpha subunit Cav3.2 Antibody, voltage-gated calcium channel alpha subunit CavT.2 Antibody, Voltage-gated calcium channel subunit alpha Cav3.2 Antibody
Research Areas Calcium Channels, Cancer, Cell Signaling, Ion Channels, Neuroscience, Voltage-Gated Calcium Channels
Cellular Localization Membrane
Accession Number NP_001005407.1
Gene ID 8912
Swiss Prot O95180
Scientific Background CaV3.2 is a protein which in humans is encoded by the CACNA1H gene. Studies suggest certain mutations in this gene lead to childhood absence epilepsy (1, 2). Studies also suggest that the up-regulations of CaV3.2 may participate in the progression of prostate cancer toward an androgen-independent stage (3).
References 1. Chen Y., et al. (2003) Ann. Neurol. 54(2): 239–43.
2. Khosravani H., et al. (2004) J Biol Chem. 279(11): 9681-9684.
3. Gackiere F., et al. (2008) J Biol Chem. 283(28): 19872.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav3.2 Monoclonal Antibody, Clone N55/10 (SMC-303). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav3.2 Monoclonal Antibody (SMC-303) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav3.2 Antibody (D) Composite.</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav3.2 Monoclonal Antibody, Clone N55/10 (SMC-303). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav3.2 Monoclonal Antibody (SMC-303) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav3.2 Antibody (D) Composite.

<p>Immunohistochemistry analysis using Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody, Clone N55/10 (SMC-303). Tissue: hippocampus. Species: Human. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody (SMC-303) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.</p>

Immunohistochemistry analysis using Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody, Clone N55/10 (SMC-303). Tissue: hippocampus. Species: Human. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody (SMC-303) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

<p>Immunohistochemistry analysis using Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody, Clone N55/10 (SMC-303). Tissue: frozen brain section. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody (SMC-303) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.</p>

Immunohistochemistry analysis using Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody, Clone N55/10 (SMC-303). Tissue: frozen brain section. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody (SMC-303) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.

<p>Western Blot analysis of Rat brain membrane lysate (native) showing detection of ~261 kDa Cav3.2 protein using Mouse Anti-Cav3.2 Monoclonal Antibody, Clone N55/10 (SMC-303). Block: 2% Skim Milk + 2% BSA in TBST. Primary Antibody: Mouse Anti-Cav3.2 Monoclonal Antibody (SMC-303) at 1:1000 for 2 hours at RT. Secondary Antibody: Anti-Mouse: HRP at 1:4000. Predicted/Observed Size: ~261 kDa.</p>

Western Blot analysis of Rat brain membrane lysate (native) showing detection of ~261 kDa Cav3.2 protein using Mouse Anti-Cav3.2 Monoclonal Antibody, Clone N55/10 (SMC-303). Block: 2% Skim Milk + 2% BSA in TBST. Primary Antibody: Mouse Anti-Cav3.2 Monoclonal Antibody (SMC-303) at 1:1000 for 2 hours at RT. Secondary Antibody: Anti-Mouse: HRP at 1:4000. Predicted/Observed Size: ~261 kDa.

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