MOLECULAR SIGNATURE®
Anti-Citrulline Antibody [6C2.1]

Mouse Anti-Citrullinated protein Monoclonal IgG1

Catalog No. SMC-501

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Species Reactivity ALL
Applications WB IHC ICC/IF FCM IP
SKU: SMC-501 Categories: ,

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SMC-501_Citrulline_Antibody_6C21_ICC-IF_Human_Embryonic-kidney-cells-HEK293_Composite_1.png
Mouse Anti-Citrulline Antibody [6C2.1] used in Immunohistochemistry (IHC) on Mouse Colon tissue (SMC-501)Mouse Anti-Citrulline Antibody [6C2.1] used in Western Blot (WB) on Citrulline-BSA Conjugate (SMC-501)
Product Name Citrulline Antibody
Description

Mouse Anti-Citrullinated protein Monoclonal IgG1

Species Reactivity Species Independent
Applications WB, ICC/IF, IHC, ELISA
Antibody Dilution WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC: (1:25) optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Synthetic L-Citrulline conjugated to Keyhole Limpet Hemocyanin (KLH).
Concentration 1 mg/ml
Conjugates APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature -20ºC, Conjugated antibodies should be stored according to the product label
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number 6C2.1
Isotype IgG1
Specificity Specific for citrulline residues on modified proteins. Tested using citrulline conjugated Bovine Serum Albumin (BSA).
Cite This Product StressMarq Biosciences Cat# SMC-501, RRID: AB_2702657
Certificate of Analysis A 1:1000 dilution of SMC-501 was sufficient for detection of Citrulline in 2 µg of Citrulline conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Biological Description

Alternative Names L-Citrulline Antibody, 2-Amino-5-(carbamoylamino)pentanoic acid, Citrulline Antibody, Citrulline Antibody, Citrulline-modified protein Antibody, ACPA, Anti-citrullinated protein antibodies, Anti-citrullinated protein Antibody, Modified Citrulline Antibody, Citrulline-containing protein Antibody, Citrullinated protein Antibody
Research Areas Cell Signaling, Citrullination, Post-translational Modifications

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1 (SMC-501). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody (SMC-501) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1 (SMC-501). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody (SMC-501) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

<p>Western Blot analysis of Citrulline-BSA Conjugate showing detection of 67 kDa Citrulline protein using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1 (SMC-501). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (2.0 µg). Lane 4: Citrulline-BSA (0.5 µg). Lane 5: Citrulline-BSA (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody (SMC-501) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.</p>

Western Blot analysis of Citrulline-BSA Conjugate showing detection of 67 kDa Citrulline protein using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1 (SMC-501). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (2.0 µg). Lane 4: Citrulline-BSA (0.5 µg). Lane 5: Citrulline-BSA (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody (SMC-501) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

<p>Immunohistochemistry analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1 (SMC-501). Tissue: Colon tissue. Species: Mouse. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody (SMC-501) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite.</p>

Immunohistochemistry analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1 (SMC-501). Tissue: Colon tissue. Species: Mouse. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody (SMC-501) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite.

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