Anti-ENaC beta Antibody [16E4]

Mouse Anti-Rat ENaC beta Monoclonal IgG2a

Catalog No. SMC-241

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Species Reactivity Ms
Applications WB IHC ICC/IF FCM IP
SKU: SMC-241D Categories: ,

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SMC-241-ENaC-beta-Antibody-16E4-WB-Mouse-Whole-kidney-homogenates-1.png
Product Name ENaC beta Antibody
Description

Mouse Anti-Rat ENaC beta Monoclonal IgG2a

Species Reactivity Mouse
Applications WB
Antibody Dilution WB (1:1000); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Rat
Immunogen Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617-638)
Concentration 1 mg/ml
Conjugates APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20ºC, Conjugated antibodies should be stored according to the product label
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number 16E4
Isotype IgG2a
Specificity Detects ~87kDa.
Cite This Product StressMarq Biosciences Cat# SMC-241, RRID: AB_2699556
Certificate of Analysis A 1:1000 dilution of SMC-241 was sufficient for detection of ENaC beta in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names SCNN1B Antibody, Amiloride sensitive sodium channel subunit beta Antibody, Amiloride-sensitive sodium channel beta-subunit Antibody, Beta ENaC Antibody, Beta NaCH Antibody, ENaC beta Antibody, ENaCB Antibody, Epithelial Na(+) channel subunit beta Antibody, Epithelial Na+ channel beta subunit Antibody, Epithelial Na+ channel subunit beta Antibody, Epithelial sodium channel beta 2 subunit Antibody, Epithelial sodium channel beta 3 subunit Antibody, Nonvoltage gated sodium channel 1 beta subunit Antibody, Nonvoltage gated sodium channel 1 subunit beta Antibody, Nonvoltage-gated sodium channel 1 beta subunit Antibody, SCNEB Antibody, SCNN 1B Antibody, Sodium channel nonvoltage gated 1 beta (Liddle syndrome) Antibody, Sodium channel nonvoltage gated 1 beta Antibody
Research Areas Epithelial Sodium Channels (ENaC), Ion Channels, Neuroscience, Sodium Channels
Cellular Localization Apical cell membrane
Accession Number NP_036780
Gene ID 24767
Swiss Prot P37090
Scientific Background The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
References 1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689.
2. Gu Y. (2008) J Cell Physiol. 216(2):453-457.
3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609.
4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594.
5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45.
6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.

Product Images

<p>Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~87kDa ENaC beta protein using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 16E4 (SMC-241). Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 µg. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody (SMC-241) at 1:1000. Predicted/Observed Size: ~87kDa.</p>

Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~87kDa ENaC beta protein using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 16E4 (SMC-241). Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 µg. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody (SMC-241) at 1:1000. Predicted/Observed Size: ~87kDa.

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