Anti-EndoPDI Antibody [2E7/7]

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Mouse Anti-Human EndoPDI Monoclonal IgG2a

Catalog No. SMC-204

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Species Reactivity Hu
Applications WB IHC ICC/IF FCM IP
SKU: SMC-204 Categories: ,

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SMC-204-EndoPDI-Antibody-2E7-7-ICC-IF-Human-Cervical-Cancer-cell-line-HeLa-60X-Composite-1.png
Product Name EndoPDI Antibody
Description

Mouse Anti-Human EndoPDI Monoclonal IgG2a

Species Reactivity Human
Applications WB, IHC, ICC/IF, IP, ELISA
Antibody Dilution WB (1:200); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Human
Immunogen Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the Human EndoPDI protein
Concentration 1 mg/ml
Conjugates APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20ºC, Conjugated antibodies should be stored according to the product label
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number 2E7/7
Isotype IgG2a
Specificity Detects ~48kDa.
Cite This Product StressMarq Biosciences Cat# SMC-204, RRID: AB_2699214
Certificate of Analysis 1 µg/ml of SMC-204 was sufficient for detection of EndoPDI in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names Thioredoxin domain containing 5; ERP46; UNQ364; MGC3178; FLJ21353; FLJ90810; thioredoxin related protein; endothelial protein disulphide isomeras Antibody
Research Areas Cell Signaling, Chaperone Proteins, Organelle Markers, Protein Trafficking
Cellular Localization Endoplasmic Reticulum, Endoplasmic reticulum lumen
Accession Number NP_001139021
Gene ID 81567
Swiss Prot Q8NBS9
Scientific Background Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia (1).
References 1. Sullivan D.C., et al. (2003) J Biol Chem. 278 (47) 47079-47088.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EndoPDI Monoclonal Antibody, Clone 2E7/7 (SMC-204). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EndoPDI Monoclonal Antibody (SMC-204) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EndoPDI Monoclonal Antibody, Clone 2E7/7 (SMC-204). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EndoPDI Monoclonal Antibody (SMC-204) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.

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