Product Name | Sodium-Iodide Symporter Antibody |
Description |
Mouse Anti-Human Sodium-Iodide Symporter Monoclonal IgG1 Kappa |
Species Reactivity | Human, Mouse, Rat |
Applications | WB, IHC, ICC/IF, AM |
Antibody Dilution | WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user. |
Host Species | Mouse |
Immunogen Species | Human |
Immunogen | Mannose binding protein and human NIS fusion (AA468-643) |
Concentration | 1 mg/ml |
Conjugates |
APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated |
Field of Use | Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only. |
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated |
Storage Temperature | -20ºC, Conjugated antibodies should be stored according to the product label |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Protein G Purified |
Clonality | Monoclonal |
Clone Number | FP5 |
Isotype | IgG1 Kappa |
Specificity | Detects ~97kDa, non-glycosylated version at 68kDa. Other minor bands associated with hNIS at 160kDa, and degradation products at ~30 kDa, and ~15kDa. |
Cite This Product | StressMarq Biosciences Cat# SMC-391, RRID: AB_10804548 |
Certificate of Analysis | 1 µg/ml of SMC-391 was sufficient for detection of hNIS in 20 µg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Alternative Names | NIS Antibody, SLC5A5 Antibody, solute carrier family 5 Antibody, Na (+)I(-) cotransporter Antibody |
Research Areas | Cancer, Cell Signaling, Ion Pumps/Transporters, Neuroscience, Pumps/Transporters |
Cellular Localization | Membrane |
Accession Number | NP_000444.1 |
Gene ID | 6528 |
Swiss Prot | Q92911 |
Scientific Background | The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells (1, 2). This is important step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine (3). |
References |
1. Dai G., Levy O., Carrasco N. (1996) Nature. 379(6564): 458-460. 2. Snabik P.A., et al. (1997) Endocrin. 138(8): 3555-3558. 3. Dohan O., et al. (2007) Proc Natl Acad Sci USA. 104(51): 20250-20255. |
Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (SMC-391). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (SMC-391) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (SMC-391). Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (SMC-391) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (SMC-391). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (SMC-391) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (SMC-391). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (SMC-391) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
StressMarq Biosciences :
Based on validation through cited publications.