Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated |
Storage Temperature | -20ºC, Conjugated antibodies should be stored according to the product label |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Protein G Purified |
Clonality | Monoclonal |
Clone Number | N245/36 (Formerly sold as S245-36) |
Isotype | IgG2a |
Specificity | Detects ~35-55kDa. Does not cross-react with TARPGamma3. |
Cite This Product | StressMarq Biosciences Cat# SMC-422, RRID: AB_2701343 |
Certificate of Analysis | 1 µg/ml of SMC-422 was sufficient for detection of TARP Gamma2/4/8 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Alternative Names | CACNG2 Antibody, Calcium channel voltage dependent gamma subunit 2 Antibody, CaV gamma 2 Antibody, Ipr328 Antibody, MGC123981 Antibody, MGC138502 Antibody, MGC138504 Antibody, Voltage-dependent calcium channel gamma-2 subunit Antibody, Neuronal voltage-gated calcium channel gamma-2 subunit Antibody, Stargazin Antibody, Stg Antibody, Stargazer Antibody, TARP Antibody, TARP gamma-2 Antibody, Transmembrane AMPAR regulatory protein gamma-2 Antibody, AW060990 Antibody, B230105C07Rik Antibody, B930041E13Rik Antibody, Wag Antibody, Waggler Antibody |
Research Areas | Calcium Channels, Cell Markers, Ion Channels, Neuron Markers, Neuroscience, Postsynaptic Markers, Voltage-Gated Calcium Channels |
Cellular Localization | Membrane |
Accession Number | NP_445803.1 |
Gene ID | 84347 |
Swiss Prot | Q71RJ2 |
Scientific Background | Stargazin belongs to a group called voltage gated calcium channels (VGCCs) which are present in most excitable cells. Stargazin is thought to stabilize the calcium channel in an inactivated (closed) state. There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival. |
References |
1. Gill M.B., et al. (2011) J Neurosci. 31(18): 6928-6938. 2. Yu L. (2011) Neuroscience. 178: 13-20. 3. Steinmetz C.C., Turrigiano G.G. (2010) J Neurosci. 30: 14685-14690. 4. Kato. A.S. et al. (2007) J Neurosci. 27: 4969-4977. 5. Letts V.A., et al. (1998) Nat Genet. 19: 340-34 |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone N245/36 (SMC-422). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (SMC-422) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody (D) Composite.
Western blot of rat brain lysate showing detection of ~55 kDa TARP Gamma2-4-8 protein using Anti-Tarp Gamma2-4-8 antibody [N245/36] (SMC-422). Lane 1: MW ladder. Lane 2: Anti-TARP Gamma2-4-8 (1:100). Lane 3: Anti-TARP Gamma2-4-8 (1:250). Lane 4: Anti-TARP Gamma2-4-8 (1:500). Lane 5: Anti-TARP Gamma2-4-8 (1:1000). Load: 20 µg RB lysate per lane. Block: 5% milk + TBST 1hr at RT. Primary antibody: Mouse Anti-TARP Gamma2-4-8 primary antibody [245-36] (SMC-422) incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody at 1:50 for 60 min at RT. Colour Development: TMB solution for 30 min at RT. Predicted/Observed size: ~ 55 kDa. Other band(s) (faint): ~37kDa, ~40kDa, ~75kDa.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone N245/36 (SMC-422). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (SMC-422) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody. (D) Composite.
StressMarq Biosciences :
Based on validation through cited publications.