Storage Buffer | PBS pH7.4, 50% glycerol, 0.1% sodium azide *Storage buffer may change when conjugated |
Storage Temperature | -20ºC, Conjugated antibodies should be stored according to the product label |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Protein G Purified |
Clonality | Monoclonal |
Clone Number | N351/23 (Formerly sold as S351-23) |
Isotype | IgG1 |
Specificity | Detects >350kDa. |
Cite This Product | StressMarq Biosciences Cat# SMC-439, RRID: AB_2701649 |
Certificate of Analysis | 1 µg/ml of SMC-439 was sufficient for detection of Versican in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
Alternative Names | Chondroitin sulfate proteoglycan 2 Antibody, CSPG2 Antibody, ERVR Antibody, GHAP Antibody, PG-M Antibody, VCAN Antibody, Chondroitin sulfate proteoglycan 2 Antibody, Chondroitin sulfate proteoglycan core protein 2 Antibody, Glial hyaluronate binding protein Antibody, Glial hyaluronate-binding protein Antibody, Large fibroblast proteoglycan Antibody, Large fibroblast proteoglycan Antibody, PGM Antibody, V1 Neo Antibody, Versican core protein Antibody, Versican proteoglycan Antibody, Versican V0 Antibody, WGN 1 Antibody, WGN Antibody, WGN1 Antibody |
Research Areas | Cell Signaling, Neuroscience, Scaffold Proteins |
Cellular Localization | Extracellular Matrix, Extracellular Space |
Accession Number | AAH96495 |
Gene ID | 13003 |
Swiss Prot | Q62059 |
Scientific Background | Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteoglycan involved in cell growth and differentiation. Important as a structural molecule, versican creates loose and hydrated matrices during key events in development and disease. The protein contains hyaluronic acid and glycosminoglycan-binding domains, epidermal growth factor-like repeats, a Lectinlike sequence and a complement regulatory protein-like domain. Splice variants differ greatly in length anddegree of modification by glycoaminoglycan chains. Accumulation around smooth muscle cells in lesions of athero-sclerosis suggests a role for versican in atherogenesis. Versican, differentially expressed in human melanoma, plays a role in tumor development and may be a reliable marker for clinical diagnosis. The organization of HA- and versican-rich pericellular matrices may faciliatate migration and mitosis by diminishing cell surface adhesivity and affecting cell shape through steric exclusion and the viscous properties of HA proteoglycan gels. |
References |
1. Dours-Zimmermann M.T. and Zimmermann D.R. (1994) J. Biol. Chem. 52: 32992-32998. 2. Evanko S.P., Angello J.C. and Wight T.N. (1999) Arterioscler. Thromb. Vasc. Biol. 4:1004-1013. 3. Lemire J.M., et al. (1999) Arterioscler. Thromb. Vasc. Biol. 7: 1630-1639. 4.Wight T.N. (2002) Curr. Opin. Cell Biol. 5: 617-623. 5. Touab M., Villena J., Barranco C., Arumi-Uria M. and Bassols A. (2002) Am. J. Pathol. 2: 549-557. |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Versican Monoclonal Antibody, Clone N351/23 (SMC-439). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (SMC-439) at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Versican Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Versican Monoclonal Antibody, Clone N351/23 (SMC-439). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (SMC-439) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Versican Antibody. (D) Composite.
Western Blot analysis of Rat Brain Membrane and brain showing detection of 350kDa Versican protein using Mouse Anti-Versican Monoclonal Antibody, Clone N351/23 (SMC-439). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Lane 3: Rat Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (SMC-439) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: KPL TMB. Predicted/Observed Size: 350kDa. Other Band(s): Multiple other bands.
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