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Anti-Aquaporin 1 Antibody
| Product Name | Aquaporin 1 Antibody |
| Description |
Rabbit Anti-Rat Aquaporin 1 Polyclonal |
| Species Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, ICC/IF |
| Antibody Dilution | WB (1:2000), IHC (1:200), ICC/IF (1:400); optimal dilutions for assays should be determined by the user. |
| Host Species | Rabbit |
| Immunogen Species | Rat |
| Immunogen | Produced against the N-terminal peptide (sequence N-MASEFKKKLF) of rat Aquaporin 1 |
| Concentration | 1 mg/ml |
| Conjugates |
APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated |
| Field of Use | Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only. |
Properties
| Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated |
| Storage Temperature | -20ºC, Conjugated antibodies should be stored according to the product label |
| Shipping Temperature | Blue Ice or 4ºC |
| Purification | Protein A purified |
| Clonality | Polyclonal |
| Specificity | Detects ~28.5kDa. May detect larger glycosylated bands ~35-50kDa. |
| Cite This Product | StressMarq Biosciences Cat# SPC-502, RRID: AB_10984185 |
| Certificate of Analysis | 0.5 µg/ml of SPC-502 was sufficient for detection of aquaporin 1 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody. |
Biological Description
| Alternative Names | AQP1 Antibody, AQP 1 Antibody, AQP CHIP Antibody, AQP-1 Antibody, AQP1 Antibody, AQP1_HUMAN Antibody, Aquaporin CHIP Antibody, Aquaporin-1 Antibody, Aquaporin-CHIP Antibody, Aquaporin1 Antibody, Channel forming integral protein 28kDa Antibody, Channel like integral membrane protein 28 kDa Antibody, CHIP 28 Antibody, CHIP28 Antibody, CO Antibody, Colton blood group Antibody, Growth factor induced delayed early response protein Antibody, MGC26324 Antibody, Urine water channel Antibody, Water channel protein CHIP 29 Antibody, Water channel protein CHIP29 Antibody, Water channel protein for red blood cells and kidney proximal tubule Antibody |
| Research Areas | Aquaporins, Ion Channels, Neuroscience, Neurotransmission |
| Cellular Localization | Membrane |
| Accession Number | NP_036910.1 |
| Gene ID | 25240 |
| Swiss Prot | P29975 |
| Scientific Background | Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquarporin 1 is a widely expressed water channel, found in the basolateral and apical plasma membranes of the proximal tubes, the descending loop of Henel and in the descending portion of the vasa recta. Additionally it is found in red blood cells, vascular endothelium, gastrointestinal tract, sweat glands and lungs. It is not regulated by vasopressin (3). |
| References |
1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396. 2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258. 3. www.vivo.colostate.edu/hbooks/molecules/aquaporins.html |
Product Images
Immunohistochemistry analysis using Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 µm cryostat sections.
Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 1 protein using Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502). Primary Antibody: Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502) at 1:2000. Showing glycosylated and non-glycosylated bands.
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Based on validation through cited publications.