Anti-CBP Antibody

Rabbit Anti-Human CBP Polyclonal

Catalog No. SPC-752

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Species Reactivity Hu
Applications WB IHC ICC/IF FCM IP
SKU: SPC-752D Categories: ,

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SPC-752_CBP_Antibody_IHC_Human_Breast-Cancer_10X_1.png
Rabbit Anti-CBP Antibody used in Western blot (WB) on Cervical cancer cell line (HeLa) lysate (SPC-752)
Product Name CBP Antibody
Description

Rabbit Anti-Human CBP Polyclonal

Species Reactivity Human
Applications WB, IHC
Antibody Dilution WB (1:1000); IHC (1:50); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthetic peptide from the mid-protein of Human CBP (aa. 2100-2200)
Concentration 1 mg/ml
Conjugates APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20ºC, Conjugated antibodies should be stored according to the product label
Shipping Temperature Blue Ice or 4ºC
Purification Peptide Affinity Purified
Clonality Polyclonal
Specificity Detects 265 kDa.
Cite This Product StressMarq Biosciences Cat# SPC-752D, RRID: AB_2728970
Certificate of Analysis A 1:1000 dilution of SPC-752 was sufficient for detection of CBP in 10 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names RSTS Antibody, CBP_HUMAN Antibody, Cyclic AMP responsive enhancer binding protein Antibody, Crebbp Antibody, CREB-binding protein Antibody, Rubinstein Taybi syndrome Antibody, KAT3A Antibody, EC:2.3.1.48 Antibody, CREBBP Antibody, CBP Antibody, RTS Antibody, CREB binding protein Antibody
Research Areas Cancer, Cancer Lipid and Lipoprotein Metabolism, Cancer Metabolism, Cell Signaling, Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Response to Hypoxia, Signaling Pathways, Stem Cells, Transcription, Wnt / Beta Catenin Pathway
Cellular Localization Cytoplasm, Nucleus
Accession Number NP_001073315.1
Gene ID 1387
Swiss Prot Q92793

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-CBP Polyclonal Antibody (SPC-752). Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-752) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.</p>

Immunohistochemistry analysis using Rabbit Anti-CBP Polyclonal Antibody (SPC-752). Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-752) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

<p>Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-752). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-752) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.</p>

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-752). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-752) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.

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