Anti-Cpn10 Antibody

Rabbit Anti-Human Cpn10 Polyclonal

Catalog No. SPC-193

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Species Reactivity Hu, Ms, Rt, Bv, Dg, GP, Pg, Rb, Sh, Xe
Applications WB IHC ICC/IF FCM IP
SKU: SPC-193 Categories: ,

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SPC-193_Cpn10_Antibody_IHC_Mouse_backskin_1.png
Rabbit Anti-Cpn10 Antibody used in Western blot (WB) on brain cell lysates (SPC-193)
Product Name Cpn10 Antibody
Description

Rabbit Anti-Human Cpn10 Polyclonal

Species Reactivity African clawed frog (Xenopus laevis), Bovine, Dog, Guinea Pig (Cavia porcellus), Human, Mouse, Pig, Rabbit, Rat, Sheep
Applications WB, IHC, ICC/IF, IP, ELISA
Antibody Dilution WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Human Cpn10 peptide AA 91-101
Concentration 1 mg/ml
Conjugates APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Buffer PBS pH7.3, 0.02% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20ºC, Conjugated antibodies should be stored according to the product label
Shipping Temperature Blue Ice or 4ºC
Purification Protein A purified
Clonality Polyclonal
Specificity Detects ~10kDa
Cite This Product StressMarq Biosciences Cat# SPC-193, RRID: AB_2119080
Certificate of Analysis 1 µg/ml of SPC-193 was sufficient for detection of Cpn10 in 10 µg of Hela lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names 10kDa Chaperonin Antibody, Chaperonin 10 Antibody, Cpn 10 Antibody, EPF Antibody, GROES Antibody, Heat Shock 10kD protein1 Antibody, HSP10 Antibody, HSPE1 Antibody
Research Areas Cancer, Cell Signaling, Chaperone Proteins, Heat Shock, Metabolism, Mitochondrial Markers, Mitochondrial Metabolism, Protein Trafficking
Cellular Localization Mitochondrion, Mitochondrion Matrix
Accession Number NP_002148.1
Gene ID 3336
Swiss Prot P61604
Scientific Background Chaperonin 10, otherwise known as Cpn10, (groES in E.coli) make up a family of small heart shock proteins with an approximate molecular mass of 10kDa (HSP10s). Cpn10 acts as a co-chaperone and interacts with the HSP60 family to promote proper folding of polypeptides. Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly process (1). Cpn10 has been located in human platelets, but is also present in human maternal serum (2, 3). It has been reported that human Cpn10 is identical with early pregnancy factor, which is involved in control over cell growth and development. This identification suggest that Cpn10 may act like a hormone in stressful situations such as pregnancy (4).
References 1. Velez-Granell C.S., et al. (1994) J of Cell Science. 107(3): 539-549.
2. Morton H., Hegh V., and Clunie G.J.A. (1974) Nature (London) 249: 459-460.
3. Cavanagh A.C., and Morton H. (1994) Eur. J. Biochem. 222: 551-560.
4. Minto M., et al. (1998) Molecular Cell Research. 1403 (2): 151-157.

Product Images

<p>Immunohistochemistry analysis using Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative Solution. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis..</p>

Immunohistochemistry analysis using Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative Solution. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis..

<p>Western blot analysis of Rat brain cell lysates showing detection of Cpn10 protein using Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.</p>

Western blot analysis of Rat brain cell lysates showing detection of Cpn10 protein using Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

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