Anti-SOD1 (EDI) Antibody

Rabbit Anti-Human SOD1 (EDI) Polyclonal

Catalog No. SPC-206

0 out of 5 based on 0 customer ratings
Species Reactivity Hu, Ms, Rt, Pg
Applications WB IHC ICC/IF FCM IP
SKU: SPC-206D Categories: ,

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CAD $436.80

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SPC-206-SOD1-EDI-Antibody-WB-Mouse-Lung-1.png
Product Name SOD1 (EDI) Antibody
Description

Rabbit Anti-Human SOD1 (EDI) Polyclonal

Species Reactivity Human, Mouse, Pig, Rat
Applications WB, IHC, ELISA
Antibody Dilution WB (1:1000); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen C-terminal region of human SOD1, exposed dimer interface (EDI)
Concentration 1 mg/ml
Conjugates APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Buffer PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20ºC, Conjugated antibodies should be stored according to the product label
Shipping Temperature Blue Ice or 4ºC
Purification Protein A purified
Clonality Polyclonal
Specificity Recognizes a conformation specific epitope where the dimer interface is exposed.
Cite This Product StressMarq Biosciences Cat# SPC-206, RRID: AB_2704217
Certificate of Analysis 1µg/ml of SPC-206 was sufficient for detection of the exposed dimer interface of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names SEDI Antibody, SOD1 EDI Antibody, Superoxide dismutase 1 Antibody, SOD Antibody, SOD1 exposed dimer interface Antibody
Research Areas ALS Disease, Cancer, Neurodegeneration, Neuroscience, Oxidative Stress
Cellular Localization Cytoplasm
Accession Number CAG46542
Gene ID 6647
Swiss Prot P00441
Scientific Background Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (2). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as SOD1 (EDI), which targets an exposed region of the dimer interface (EDI) of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with SOD1 (UβB) (SPC-205D) which detects an unfolded beta barrel (UβB) of SOD1.
References 1. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180.
2. Furukawa Y., and O'Halloran T. (2006) Antioxid Redox Signal. 8(5-6):847-67.
3. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol. 284:L917-L925.
4. Hassan H.M. (1988) Free Radical Biol. Med. 5:377-385.
5. Kerman A., et al. (2010) Acta Neuropathol. 119:335-344.

Product Images

<p>Western blot analysis of Mouse Lung showing detection of ~18 kDa SOD1 (EDI) protein using Rabbit Anti-SOD1 (EDI) Polyclonal Antibody (SPC-206). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Lung. Load: 20 µg. Primary Antibody: Rabbit Anti-SOD1 (EDI) Polyclonal Antibody (SPC-206) at 1:1000. Predicted/Observed Size: ~18 kDa.</p>

Western blot analysis of Mouse Lung showing detection of ~18 kDa SOD1 (EDI) protein using Rabbit Anti-SOD1 (EDI) Polyclonal Antibody (SPC-206). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Lung. Load: 20 µg. Primary Antibody: Rabbit Anti-SOD1 (EDI) Polyclonal Antibody (SPC-206) at 1:1000. Predicted/Observed Size: ~18 kDa.

Reviews

Reviews

  1. 3 out of 5

    :

    Based on recent publications (Tokuda, E. et al. (2017) Mol Neurodegener. 12(1):2.), the Anti-SOD1 (EDI) Antibody sold by StressMarq, Catalog No. SPC-206 appears to be similar to the SEDI antibody reported in Rakhit et al., Nature Medicine 13, 754 – 759 (2007).”

  2. 5 out of 5

    :

    Based on validation through cited publications.

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