Anti-Tau 2N Antibody

Rabbit Anti-Human Tau Polyclonal

Catalog No. SPC-813

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Species Reactivity Hu
Applications WB IHC ICC/IF FCM IP
SKU: SPC-813 Categories: ,

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SPC-813_Tau-2N_Antibody_DB_Human_Recombinant-Tau-Monomers_1.png
Rabbit Anti-Tau 2N Antibody used in Western blot (WB) on Human Recombinant Tau Monomers (SPC-813)Rabbit Anti-Tau 2N Antibody used in Dot blot (DB) on Mouse, Human Recombinant Tau Monomers and Fibrils (SPC-813)
Product Name Tau 2N Antibody
Description

Rabbit Anti-Human Tau Polyclonal
Species Reactivity Human
Applications WB
Antibody Dilution WB (1:1000); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Tau 2N3R/4R peptide
Concentration 1mg/mL
Conjugates APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated
Dylight 488
Overview:

  • High fluorescence yield
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 1011 g/mol

Dylight 488 Datasheet

 Dylight 488 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 493 nm

λem = 518 nm

εmax = 7.0×104

Laser = 488 nm

 

APC/Cy7
Overview:

  • High quantum yield
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested

APC-Cy7 Datasheet

 

 ACP-Cy7 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 652 nm

λem = 790 nm

Laser = 594 or 633 nm

 

 

  Dylight 350
Overview:

  • High fluorescence intensity
  • High photostability
  • Less pH-sensitive
  • Excellent solubility in water
  • Stringently QC tested
  • Excellent batch-to-batch reproducibility
  • Molecular weight: 874 g/mol

Dylight 350 Datasheet

 Dylight 350 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 353 nm

λem = 432 nm

εmax = 1.5×104

 

 

  Dylight 405
Overview:

  • High fluorescence intensity
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 793 g/mol

Dylight 405 Datasheet

 Dylight 405 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 400 nm

λem = 420 nm

εmax = 3.0×104

Laser = 405 nm

 

Dylight 594
Overview:

  • High fluorescence yield
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 1078 g/mol

Dylight 594 Datasheet

 Dylight 594 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 593 nm

λem = 618 nm

εmax = 8.0×104

Laser = 526 nm

 

 Dylight 633
Overview:

  • High fluorescence yield
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 1066 g/mol

Dylight 633 Datasheet

 Dylight 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 638 nm

λem = 658 nm

εmax = 1.7×105

Laser = 633 nm

 

 PerCP 
Overview:

  • Peridinin-Chlorophyll-Protein Complex
  • Small phycobiliprotein
  • Isolated from red algae
  • Large stokes shift (195 nm)
  • Molecular Weight: 35 kDa

PerCP Datasheet

  PerCP Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 482 nm

λem = 677 nm

εmax = 1.96 x 106

Laser = 488 nm

 

 PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation

PE/ATTO 594 Datasheet

 PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra Optical Properties:

λex = 535 nm

λem = 627 nm

Laser = 488 to 561 nm

 

  FITC (Fluorescein)
Overview:

  • Excellent fluorescence quantum yield
  • High rate of photobleaching
  • Good solubility in water
  • Broad emission spectrum
  • pH dependent spectra
  • Molecular formula: C20H12O5
  • Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

 FITC Fluorescein Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 494 nm

λem = 520 nm

εmax = 7.3×104

Φf = 0.92

τfl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

 

 ATTO 700
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 575 g/mol

ATTO 700 Datasheet

  ATTO 700 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 700 nm

λem = 719 nm

εmax = 1.25×105

Φf = 0.25

τfl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

 

 ATTO 680
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 631 g/mol

ATTO 680 Datasheet

  ATTO 680 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 680 nm

λem = 700 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

 

 ATTO 655
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Excellent ozone resistance
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 634 g/mol

ATTO 655 Datasheet

 ATTO 655 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 663 nm

λem = 684 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

 

 ATTO 633
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Stable at pH 4 – 11
  • Cationic dye, perchlorate salt
  • Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

 ATTO 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 629 nm

λem = 657 nm

εmax = 1.3×105

Φf = 0.64

τfl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

 

 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

  ATTO 594 Fluorophore Excitation and Emission Spectrum Optical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

 ATTO 565
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Good solubility in polar solvents
  • Excellent solubility in water
  • Very little aggregation
  • Rhodamine dye derivative
  • Molar Mass: 611 g/mol

ATTO 565 Datasheet

  ATTO 565 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 563 nm

λem = 592 nm

εmax = 1.2×105

Φf = 0.9

τfl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

 

  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol 

ATTO 488 Datasheet

   ATTO 488 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

  ATTO 390
Overview:

  • High fluorescence yield
  • Large Stokes-shift (89 nm)
  • Good photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Coumarin derivate, uncharged
  • Low molar mass: 343.42 g/mol 

ATTO 390 Datasheet

 ATTO 390 Fluorescent Dye Excitation and Emission Spectra Optical Properties:

λex = 390 nm

λem = 479 nm

εmax = 2.4×104

Φf = 0.90

τfl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

 

APC (Allophycocyanin)
Overview:

  • High quantum yield
  • Large phycobiliprotein
  • 6 chromophores per molecule
  • Isolated from red algae
  • Molecular Weight: 105 kDa

APC Datasheet

  APC Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 650 nm

λem = 660 nm

εmax = 7.0×105

Φf = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

 

Streptavidin

Properties:

  • Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
  • Molecular weight: 53 kDa
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

BiotinBiotin Conjugate Structure

Properties:

  • Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
  • Molar mass: 244.31 g/mol
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

HRP (Horseradish peroxidase)

Properties:

  • Enzymatic activity is used to amplify weak signals and increase visibility of a target
  • Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
    • Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
    • Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
  • High turnover rate enables rapid generation of a strong signal
  • 44 kDa glycoprotein
  • Extinction coefficient: 100 (403 nm)
  • Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

AP (Alkaline Phosphatase)

Properties:

  • Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
  • Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
    • Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
  • Molecular weight: 140 kDa
  • Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

  R-PE (R-Phycoerythrin)
Overview:

  • Broad excitation spectrum
  • High quantum yield
  • Photostable
  • Member of the phycobiliprotein family
  • Isolated from red algae
  • Excellent solubility in water
  • Molecular Weight: 250 kDa

R-PE Datasheet

  R-PE Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 565 nm

λem = 575 nm

εmax = 2.0×106

Φf = 0.84

Brightness = 1.68 x 103

Laser = 488 to 561 nm

Filter set = TRITC

 
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Storage Temperature -20ºC, Conjugated antibodies should be stored according to the product label
Shipping Temperature Blue Ice or 4ºC
Purification Affinity Purified
Clonality Polyclonal
Specificity Detects Tau 2N3R/4R spliceforms
Cite This Product Rabbit Anti-Human Tau 2N Polyclonal (StressMarq Biosciences, Victoria BC, Cat# SPC-813)
Certificate of Analysis A 1:1000 dilution of SPC-813 was sufficient for detection of Tau 2N3R/4R spliceforms (range tested 2ug – 0.063ug).

Biological Description

Alternative Names Tau, 2N4R Tau, 2N3R Tau, pTau, MAPT, Neurofibrillary tangle protein, Tau-Human
Research Areas ALS Disease, Alzheimer's Disease, Neurodegeneration, Neuroscience, Parkinson's Disease
Cellular Localization Plasma Membrane
Swiss Prot P10636-5
Scientific Background Tau is a microtubule-associated protein (MAPT) with multiple isoforms due to alternative mRNA splicing (1,2). The 2N isoform contains two N-terminal inserts and is expressed in both 3R (2N3R) and 4R (2N4R) variants (2). The 3R/4R ratio varies across diseases; for example, Alzheimer’s disease features a mixed 3R/4R pathology (3). Our human Tau 2N3R/4R polyclonal antibody has been demonstrated to be specific to only human 2N isoforms and does not detect rodent isoforms.
References 1.,Goedert, M., Spillantini, M. G., Jakes, R., Rutherford, D., & Crowther, R. A. (1989). Multiple isoforms of human microtubule-associated protein tau: sequences and localization in neurofibrillary tangles of Alzheimer's disease. Neuron, 3(4), 519–526. DOI: 10.1016/0896-6273(89)90210-9
2.,Spillantini, M. G., & Goedert, M. (2013). Tau pathology and neurodegeneration. The Lancet Neurology, 12(6), 609–622. DOI: 10.1016/S1474-4422(13)70090-5
3.,Kovacs, G. G. (2017). Tauopathies. Handbook of Clinical Neurology, 145, 355–368. DOI: 10.1016/B978-0-12-802395-2.00025-3

Product Images

<p>Dot blot analysis comparing detection of Tau isoforms. Of note is that SPC-813 does not bind to 0N or 1N isoforms. Block: 5% skim milk. Primary Antibody: Anti-human Tau 2N3R/4R polyclonal antibody (SPC-813) incubated for 1/2 hour at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min in RT. Exposed 1 second.</p>

Dot blot analysis comparing detection of Tau isoforms. Of note is that SPC-813 does not bind to 0N or 1N isoforms. Block: 5% skim milk. Primary Antibody: Anti-human Tau 2N3R/4R polyclonal antibody (SPC-813) incubated for 1/2 hour at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min in RT. Exposed 1 second.

<p>Western blot analysis comparing detection of Tau isoforms, human Tau 0N3R monomer (SPR-490), human Tau 1N3R monomer (SPR-513), and human Tau 2N3R monomer. Of note is that SPC-813 does not bind to the 0N or 1N isoforms. Block: 5% skim milk. Primary Antibody: Anti-human Tau 2N3R/4R polyclonal antibody (SPC-813) incubated for 1/2 hour at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min in RT. Exposed 1 second.</p>

Western blot analysis comparing detection of Tau isoforms, human Tau 0N3R monomer (SPR-490), human Tau 1N3R monomer (SPR-513), and human Tau 2N3R monomer. Of note is that SPC-813 does not bind to the 0N or 1N isoforms. Block: 5% skim milk. Primary Antibody: Anti-human Tau 2N3R/4R polyclonal antibody (SPC-813) incubated for 1/2 hour at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min in RT. Exposed 1 second.

<p>Dot blot analysis comparing detection of mouse Tau 2N4R monomer and human Tau 2N4R pre-formed fibrils. Of note is that SPC-813 does not bind to the mouse construct. Block: 5% skim milk. Primary Antibody: Anti-human Tau 2N3R/4R polyclonal antibody (SPC-813) incubated for 1/2 hour at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min in RT. Exposed 1 second.</p>

Dot blot analysis comparing detection of mouse Tau 2N4R monomer and human Tau 2N4R pre-formed fibrils. Of note is that SPC-813 does not bind to the mouse construct. Block: 5% skim milk. Primary Antibody: Anti-human Tau 2N3R/4R polyclonal antibody (SPC-813) incubated for 1/2 hour at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min in RT. Exposed 1 second.

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