Anti-TbHsp70 Antibody

Rabbit Anti-Trypanosoma brucei brucei TbHsp70 Polyclonal

Catalog No. SPC-747

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Species Reactivity Tb
Applications WB IHC ICC/IF FCM IP
SKU: SPC-747D Categories: ,

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USD $337.00

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SPC-747_TbHsp70_Antibody_WB_Trypanosoma-brucei-brucei_cells_1.png
Product Name TbHsp70 Antibody
Description

Rabbit Anti-Trypanosoma brucei brucei TbHsp70 Polyclonal

Species Reactivity Trypanosoma brucei brucei
Applications WB
Antibody Dilution WB (1:1000); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Trypanosoma brucei brucei
Immunogen Synthetic peptide from the mid-protein of Trypanosoma brucei brucei TbHsp70 (aa. 200-300)
Concentration 1 mg/ml
Conjugates APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20ºC, Conjugated antibodies should be stored according to the product label
Shipping Temperature Blue Ice or 4ºC
Purification Peptide Affinity Purified
Clonality Polyclonal
Specificity Detects ~71 kDa.
Cite This Product StressMarq Biosciences Cat# SPC-747D, RRID: AB_2728965
Certificate of Analysis A 1:1000 dilution of SPC-747 was sufficient for detection of TbHsp70 in 15 µg of trypanosoma brucei brucei cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names TbHsp70 Antibody, Heat shock 70 kDa protein 4 Antibody, HSP70-4 Antibody
Research Areas Cancer, Cell Signaling, Chaperone Proteins, Heat Shock, Protein Trafficking, Tumor Biomarkers
Swiss Prot P11145

Product Images

<p>Western blot analysis of Trypanosoma brucei brucei cells showing detection of ~71 kDa TbHsp70 protein using Rabbit Anti-TbHsp70 Polyclonal Antibody (SPC-747). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cells incubated for 1 hour at 37°C. Lane 3: Cells incubated for 1 hour at 42°C. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TbHsp70 Polyclonal Antibody (SPC-747) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71 kDa.</p>

Western blot analysis of Trypanosoma brucei brucei cells showing detection of ~71 kDa TbHsp70 protein using Rabbit Anti-TbHsp70 Polyclonal Antibody (SPC-747). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cells incubated for 1 hour at 37°C. Lane 3: Cells incubated for 1 hour at 42°C. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TbHsp70 Polyclonal Antibody (SPC-747) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71 kDa.

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