Storage Buffer | PBS pH 7.4, 50% glycerol, 0.09% sodium azide. *Storage buffer may change when conjugated |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Protein A purified |
Clonality | Recombinant Monoclonal |
Clone Number | 2F9 |
Isotype | IgG |
Specificity | Amyloid Beta 1-42 oligomers |
Cite This Product | Rabbit Anti-Human Amyloid Beta 1-42 Oligomer Recombinant Monoclonal (StressMarq Biosciences, Victoria BC, Cat# SMC-620) |
Certificate of Analysis | A 1:10,000 dilution of SMC-620 was sufficient for detection of 1ug of AB 1-42 oligomers by western blot analysis with goat-anti-rabbit:HRP as the secondary and ELISA analysis (capture at 1 ug/mL, 6E10:HRP used as the detection at 0.25ug/mL). |
Alternative Names | Abeta Oligomers Antibody, APP Antibody |
Research Areas | Alzheimer's Disease, Amyloid, Neurodegeneration, Neuroscience |
Cellular Localization | Membrane (Secreted) |
Gene ID | 351 (APP) |
Swiss Prot | P05067 |
Scientific Background | Our amyloid beta oligomer antibody (clone 2F9) has been tested for specificity against synthetic AB 1-42 aggregates, with higher specificity to oligomers demonstrated. In the brain, amyloid beta peptide (Aβ) is generated by protease cleavage of amyloid precursor protein (APP), which aggregates into oligomers, protofibrils, fibrils and ultimately plaques in neurodegenerative diseases. The accumulation of Aβ plaques in the brain is considered a hallmark of Alzheimer’s disease (AD), and most of the drugs tested for AD in the past 20 years have targeted amyloid beta accumulation (1). The immunogen used was our Amyloid Beta 1-42 (Aβ42) Oligomers are generated from Amyloid Beta Peptide 1-42 pre-treated with 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP) as previously published (2,3). |
References |
1. Panza et al. 2019. Nat Rev Neurol. 15:73-88 https://doi.org/10.1038/s41582-018-0116-6 2. Stine et al. 2003. JBC. 278(13):11612-22. doi: 10.1074/jbc.M210207200 3. Ahmed et al. 2010. Nature Structural & Molecular Biology. 17(5):561 doi: 10.1038/nsmb.1799 |
Western blot analysis comparing detection of StressMarq’s Amyloid Beta 1-42 constructs (SPR-485 = amyloid beta 1-42 peptide; SPR-488 = amyloid beta 1-42 oligomers; SPR-487 = amyloid beta 1-42 pre-formed fibrils; SPR-492 = amyloid beta pyroglutamate 3-42 pre-formed fibrils). Block: 5% skim milk. Primary Antibodies: Rabbit Anti-Amyloid Beta 1-42 oligomer monoclonal antibody (SMC-620, clone 2F9) & Mouse Anti-Amyloid Beta 1-16 monoclonal antibody (Biolegend 803001, clone 6E10), at 1:10,000 & 1:1000, respectively, for 1/2 hour at RT. Secondary Antibodies: Goat anti-rabbit IgG:HRP or anti-mouse IgG:HRP at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min in RT. Exposed 1 second.
Dot blot analysis comparing detection of StressMarq’s amyloid beta 1-42 constructs, alpha synuclein wildtype constructs, and tau constructs. Of note is that SMC-620 does not bind APP 751 or other protein constructs such as alpha synuclein and tau. Block: 5% skim milk. Primary Antibodies: Anti-Amyloid Beta 1-42 oligomer monoclonal antibody (SMC-620, clone 2F9), Anti-Amyloid Beta 1-16 monoclonal antibody (Biolegend 803001, clone 6E10), Anti-Alpha Synuclein polyclonal antibody (SPC-800), and Anti-Tau polyclonal antibody (SPC-801), all incubated for 1/2 hour at RT. Secondary Antibodies: Goat anti-rabbit IgG:HRP or anti-mouse IgG:HRP at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min in RT. Exposed 1 second.
Dot blot analysis comparing detection of StressMarq’s amyloid beta constructs. Concentrations tested on each blot are 20 & 2ng, respectively. Block: 5% skim milk. Primary Antibodies: Rabbit Anti-Amyloid Beta 1-42 oligomer monoclonal antibody (SMC-620, clone 2F9) & Mouse Anti-Amyloid Beta 1-16 monoclonal antibody (Biolegend 803001, clone 6E10) at 1:10,000 & 1:1000, respectively, for 1/2 hour at RT. Secondary Antibodies: Goat anti-rabbit IgG:HRP or anti-mouse IgG:HRP at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min in RT. Exposed 1 second.
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