Storage Buffer | PBS pH 7.4, 0.02% Proclin 300, 50% glycerol |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | AmMagTM Ultra AT Protein A MagBeads |
Clonality | Recombinant Monoclonal |
Clone Number | S22 |
Isotype | IgG Kappa |
Specificity | Detects C-terminus of Slo1, epitope mapped to within AA #690-715 (PKLMRHDPLLIPGNDQIDNMDSNVKK). |
Cite This Product | Rabbit Anti-Mouse Slo1 Recombinant Monoclonal (StressMarq Biosciences, Victoria BC, Cat# SMC-625) |
Certificate of Analysis | 1 µg/ml was sufficient for detection of Slo1 on Rat Brain Membrane lysate. |
Alternative Names | Slo1/BKAlpha maxi-K+ channel Antibody, Calcium-activated potassium channel subunit alpha-1 Antibody, Calcium-activated potassium channel Antibody, Subfamily M subunit alpha-1 Antibody, KCa1.1 Antibody, K(VCA)alpha Antibody, BK channel Antibody, BKCA alpha Antibody, Maxi K channel Antibody, Slo-alpha Antibody, Slowpoke homolog Antibody, Kcnma1 Antibody, Kcnma Antibody |
Research Areas | Calcium-Activated Potassium Channels, Cancer, Cardiovascular System, Cell Signaling, Ion Channels, Neuroscience, Potassium Channels |
Cellular Localization | Cell Membrane, Nucleus |
Accession Number | AAA39746.1 |
Gene ID | 16531 |
Swiss Prot | Q08460 |
Scientific Background | The Slo1/BK Alpha maxi-K+ channel protein is predominantly found in neurons and smooth muscle, is regulated by both calcium concentrations and voltage, and is a homolog to the pH and voltage-regulated Slo3 (1,2). Slo1/BK has been shown to facilitate the repolarization phase of action potentials and modulate neurotransmitter release (3,4). Dysregulation of Slo1 channels has been linked to several disorders such as hypertension, epilepsy, ataxia (2) and muscle dystrophy (5). Our anti-slo1/BK Alpha maxi-K+ channel antibody can be used to detect the presence of this protein in various tissues. |
References |
1.,Yang, C.T., Zeng X.H., Xia X.M., and Lingle C.J. (2009). Interactions between β Subunits of the KCNMB Family and Slo3: β4 Selectively Modulates Slo3 Expression and FunctionPloS One. 4(7): e6135. DOI: 10.1371/journal.pone.0006135 2.,Latorre, R., & Brauchi, S. (2006). Large conductance Ca2+-activated K+ (BK) channel: Activation by Ca2+ and voltage. Biological Research, 39(3), 385-401. DOI: 10.4067/s0716-97602006000300003 3.,Petkov G. V. (2014). Central role of the BK channel in urinary bladder smooth muscle physiology and pathophysiology. American journal of physiology. Regulatory, integrative and comparative physiology, 307(6), R571–R584. DOI: 10.1152/ajpregu.00142.2014 4.,Pathak, D., Guan, D., & Foehring, R. C. (2016). Roles of specific Kv channel types in repolarization of the action potential in genetically identified subclasses of pyramidal neurons in mouse neocortex. Journal of neurophysiology, 115(5), 2317–2329. DOI: 10.1152/jn.01028.2015 5.,Kim, H., Pierce-Shimomura, J. T., Oh, H. J., Johnson, B. E., Goodman, M. B., & McIntire, S. L. (2009). The dystrophin complex controls bk channel localization and muscle activity in Caenorhabditis elegans. PLoS genetics, 5(12), e1000780. DOI: 10.1371/journal.pgen.1000780 |
Western blot analysis with Stressmarq’s Rabbit Anti- Slo1/BKAlpha maxi-K+ channel Monoclonal Antibody, Clone S22 (SMC-625) showing detection of Human Slo1 protein in a transient over-expression (OE) lysate (OriGene LY400815). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Mouse Slo1/BKAlpha maxi-K+ channel Recombinant Monoclonal [S22] (SMC-625) at 1:1000 for 2 hours at RT. Secondary Antibody: , at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 30 sec at RT in the dark. Exposed 15 seconds. NOTE: The ORF clone contains a C-term DDK tag; results were confirmed using Anti-DDK antibody (OriGene TA592569), showing laddering above target is related to homooligomerization of the Slo1 protein (data not shown).
Immunocytochemistry/Immunofluorescence analysis with Stressmarq’s Rabbit Anti- Slo1/BKAlpha maxi-K+ channel Monoclonal Antibody, Clone S22 (SMC-625). Tissue: Neuroblastoma cells (Kelly). Species: Human. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Mouse Slo1/BKAlpha maxi-K+ channel Recombinant Monoclonal [S22] (SMC-625) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1:1000 for 1 hour at RT in the dark. Counterstain: DAPI (blue) nuclear stain at 1:1000 for 5 min at RT in the dark.
Immunohistochemistry analysis with Stressmarq’s Rabbit Anti- Slo1/BKAlpha maxi-K+ channel Monoclonal Antibody, Clone S22 (SMC-625). Tissue: Brain. Species: Mouse. Fixation: Paraffin embedded, 4um thin. Blocking: 3% milk for 1 hour at RT. Primary Antibody: Rabbit Anti-Mouse Slo1/BKAlpha maxi-K+ channel Recombinant Monoclonal [S22] (SMC-625) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP(H&L) at 1:100 for 45 min at RT. Counterstain: Hematoxylin (purple) nuclear stain at 1:10 for 5 min at RT. Color Development: DAB at 1:50 for 15 min at RT.
Immunocytochemistry/Immunofluorescence analysis with Stressmarq’s Rabbit Anti- Slo1/BKAlpha maxi-K+ channel Monoclonal Antibody, Clone S22 (SMC-625). Tissue: Pheochromocytoma cells (PC-12). Species: Rat. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Mouse Slo1/BKAlpha maxi-K+ channel Recombinant Monoclonal [S22] (SMC-625) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1:1000 for 1 hour at RT in the dark. Counterstain: DAPI (blue) nuclear stain at 1:1000 for 5 min at RT in the dark.
Western blot analysis with Stressmarq’s Rabbit Anti-Slo1/BKAlpha maxi-K+ channel Monoclonal Antibody, Clone S22 (SMC-625) showing detection of Rat Slo1 protein in a Rat brain membrane lysate. Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Mouse Slo1/BKAlpha maxi-K+ channel Recombinant Monoclonal [S22] (SMC-625) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min at RT in the dark. Exposed 6.5 seconds.
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