Anti-Tau Antibody (pSer202/ pThr205) [AH36]

Rabbit Anti-Human Tau Monoclonal IgG

Catalog No. SMC-601

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Species Reactivity Hu, Ms
Applications WB IHC ICC/IF FCM IP
SKU: SMC-601 Categories: ,

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SMC-601_Tau_Antibody_AH36_ICC-IF_Human_iPSC-derived-cortical-excitatory-neurons_1.png
Rabbit Anti-Tau Antibody (pSer202/ pThr205) [AH36] used in Immunohistochemistry (IHC) on Mouse Brain slice (SMC-601)Rabbit Anti-Tau Antibody (pSer202/ pThr205) [AH36] used in Immunohistochemistry (IHC) on Mouse Brain slice (SMC-601)Rabbit Anti-Tau Antibody (pSer202/ pThr205) [AH36] used in Western Blot (WB) on Human iPSC-derived cortical neurons (SMC-601)Rabbit Anti-Tau Antibody (pSer202/ pThr205) [AH36] used in Dot Blot (DB) on E. Coli, Baculovirus (SMC-601)
Product Name Tau Antibody (pSer202/ pThr205)
Description

Rabbit Anti-Human Tau Monoclonal IgG
Species Reactivity Human, Mouse
Applications WB, DB, ICC/IF, ELISA, IHC
Antibody Dilution WB (1:500), ICC/IF (1:500); IHC (1:500); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthetic peptide of Human Phospho Tau (Ser202/Thr205)
Concentration 1 mg/ml
Conjugates APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated
Dylight 488
Overview:

  • High fluorescence yield
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 1011 g/mol

Dylight 488 Datasheet

 Dylight 488 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 493 nm

λem = 518 nm

εmax = 7.0×104

Laser = 488 nm

 

APC/Cy7
Overview:

  • High quantum yield
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested

APC-Cy7 Datasheet

 

 ACP-Cy7 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 652 nm

λem = 790 nm

Laser = 594 or 633 nm

 

 

  Dylight 350
Overview:

  • High fluorescence intensity
  • High photostability
  • Less pH-sensitive
  • Excellent solubility in water
  • Stringently QC tested
  • Excellent batch-to-batch reproducibility
  • Molecular weight: 874 g/mol

Dylight 350 Datasheet

 Dylight 350 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 353 nm

λem = 432 nm

εmax = 1.5×104

 

 

  Dylight 405
Overview:

  • High fluorescence intensity
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 793 g/mol

Dylight 405 Datasheet

 Dylight 405 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 400 nm

λem = 420 nm

εmax = 3.0×104

Laser = 405 nm

 

Dylight 594
Overview:

  • High fluorescence yield
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 1078 g/mol

Dylight 594 Datasheet

 Dylight 594 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 593 nm

λem = 618 nm

εmax = 8.0×104

Laser = 526 nm

 

 Dylight 633
Overview:

  • High fluorescence yield
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 1066 g/mol

Dylight 633 Datasheet

 Dylight 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 638 nm

λem = 658 nm

εmax = 1.7×105

Laser = 633 nm

 

 PerCP 
Overview:

  • Peridinin-Chlorophyll-Protein Complex
  • Small phycobiliprotein
  • Isolated from red algae
  • Large stokes shift (195 nm)
  • Molecular Weight: 35 kDa

PerCP Datasheet

  PerCP Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 482 nm

λem = 677 nm

εmax = 1.96 x 106

Laser = 488 nm

 

 PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation

PE/ATTO 594 Datasheet

 PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra Optical Properties:

λex = 535 nm

λem = 627 nm

Laser = 488 to 561 nm

 

  FITC (Fluorescein)
Overview:

  • Excellent fluorescence quantum yield
  • High rate of photobleaching
  • Good solubility in water
  • Broad emission spectrum
  • pH dependent spectra
  • Molecular formula: C20H12O5
  • Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

 FITC Fluorescein Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 494 nm

λem = 520 nm

εmax = 7.3×104

Φf = 0.92

τfl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

 

 ATTO 700
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 575 g/mol

ATTO 700 Datasheet

  ATTO 700 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 700 nm

λem = 719 nm

εmax = 1.25×105

Φf = 0.25

τfl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

 

 ATTO 680
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 631 g/mol

ATTO 680 Datasheet

  ATTO 680 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 680 nm

λem = 700 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

 

 ATTO 655
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Excellent ozone resistance
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 634 g/mol

ATTO 655 Datasheet

 ATTO 655 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 663 nm

λem = 684 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

 

 ATTO 633
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Stable at pH 4 – 11
  • Cationic dye, perchlorate salt
  • Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

 ATTO 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 629 nm

λem = 657 nm

εmax = 1.3×105

Φf = 0.64

τfl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

 

 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

  ATTO 594 Fluorophore Excitation and Emission Spectrum Optical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

 ATTO 565
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Good solubility in polar solvents
  • Excellent solubility in water
  • Very little aggregation
  • Rhodamine dye derivative
  • Molar Mass: 611 g/mol

ATTO 565 Datasheet

  ATTO 565 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 563 nm

λem = 592 nm

εmax = 1.2×105

Φf = 0.9

τfl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

 

  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol 

ATTO 488 Datasheet

   ATTO 488 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

  ATTO 390
Overview:

  • High fluorescence yield
  • Large Stokes-shift (89 nm)
  • Good photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Coumarin derivate, uncharged
  • Low molar mass: 343.42 g/mol 

ATTO 390 Datasheet

 ATTO 390 Fluorescent Dye Excitation and Emission Spectra Optical Properties:

λex = 390 nm

λem = 479 nm

εmax = 2.4×104

Φf = 0.90

τfl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

 

APC (Allophycocyanin)
Overview:

  • High quantum yield
  • Large phycobiliprotein
  • 6 chromophores per molecule
  • Isolated from red algae
  • Molecular Weight: 105 kDa

APC Datasheet

  APC Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 650 nm

λem = 660 nm

εmax = 7.0×105

Φf = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

 

Streptavidin

Properties:

  • Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
  • Molecular weight: 53 kDa
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

BiotinBiotin Conjugate Structure

Properties:

  • Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
  • Molar mass: 244.31 g/mol
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

HRP (Horseradish peroxidase)

Properties:

  • Enzymatic activity is used to amplify weak signals and increase visibility of a target
  • Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
    • Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
    • Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
  • High turnover rate enables rapid generation of a strong signal
  • 44 kDa glycoprotein
  • Extinction coefficient: 100 (403 nm)
  • Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

AP (Alkaline Phosphatase)

Properties:

  • Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
  • Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
    • Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
  • Molecular weight: 140 kDa
  • Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

  R-PE (R-Phycoerythrin)
Overview:

  • Broad excitation spectrum
  • High quantum yield
  • Photostable
  • Member of the phycobiliprotein family
  • Isolated from red algae
  • Excellent solubility in water
  • Molecular Weight: 250 kDa

R-PE Datasheet

  R-PE Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 565 nm

λem = 575 nm

εmax = 2.0×106

Φf = 0.84

Brightness = 1.68 x 103

Laser = 488 to 561 nm

Filter set = TRITC

 
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature -20ºC, Conjugated antibodies should be stored according to the product label
Shipping Temperature Blue Ice or 4ºC
Purification Affinity Purified
Clonality Monoclonal
Clone Number AH36
Isotype IgG
Specificity Detects ~79 kDa.
Cite This Product Rabbit Anti-Human Tau Monoclonal (StressMarq Biosciences, Victoria BC, Cat# SMC-601D)
Certificate of Analysis A 1:500 dilution of SMC-601 was sufficient for detection of Tau in 10 µg Baculovirus by dot blot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names Tau Antibody, Neurofibrillary tangle protein Antibody, MAPTL Antibody, Microtubule-associated protein tau Antibody, MTBT1 Antibody, Paired helical filament-tau Antibody, TAU Antibody, PHF-tau Antibody, MAPT Antibody, AI413597 antibody, AW045860 antibody, DDPAC antibody, FLJ31424 antibody, FTDP 17 antibody, G protein beta1/gamma2 subunit interacting factor 1 antibody, MAPT antibody, MAPTL antibody, MGC134287 antibody, MGC138549 antibody, MGC156663 antibody, Microtubule associated protein tau antibody, Microtubule associated protein tau isoform 4 antibody, Microtubule-associated protein tau antibody, MSTD antibody, Mtapt antibody, MTBT1 antibody, MTBT2 antibody, Neurofibrillary tangle protein antibody, Paired helical filament tau antibody, Paired helical filament-tau antibody, PHF tau antibody, PHF-tau antibody, PPND antibody, PPP1R103 antibody, Protein phosphatase 1, regulatory subunit 103 antibody, pTau antibody, RNPTAU antibody, TAU antibody, TAU_HUMAN antibody, Tauopathy and respiratory failure, included antibody, AT8 antibody, phospho tau antibody
Research Areas Alzheimer's Disease, Axon Markers, Cell Markers, Cell Signaling, Cytoskeleton, Microtubules, MT Associated Proteins, Neurodegeneration, Neuron Markers, Neuroscience, Tangles & Tau
Cellular Localization Axon, Cytoskeleton, Cytosol, Dendrite, Plasma Membrane
Gene ID 4137
Swiss Prot P10636
Scientific Background Alzheimer’s Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). Phosphorylation at serine 202 and threonine 205 induces a turn-like structure and drives tau aggregation (4). SMC-601 recognizes the same epitope as the AT8 phospho tau antibody.
References 1. www.alz.org/alzheimers-dementia/facts-figures
2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907)
3. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497.
4. Depres, C. et al. (2017). PNAS 114(34):9080-9085.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (SMC-601). Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (SMC-601) at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 μm stacks.. Courtesy of: Francesco Paonessa.</p>

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (SMC-601). Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (SMC-601) at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 μm stacks.. Courtesy of: Francesco Paonessa.

<p>Dot Blot analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (SMC-601). Species: E. Coli, Baculovirus. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (SMC-601) at 1:500. Secondary Antibody: Goat anti-rabbit IgG:HRP.</p>

Dot Blot analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (SMC-601). Species: E. Coli, Baculovirus. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (SMC-601) at 1:500. Secondary Antibody: Goat anti-rabbit IgG:HRP.

<p>Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (SMC-601). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. . Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (SMC-601) at 1:500 for Overnight. pSer202/pThr205 was detected using SMC-601. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.. Courtesy of: Francesco Paonessa.</p>

Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (SMC-601). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. . Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (SMC-601) at 1:500 for Overnight. pSer202/pThr205 was detected using SMC-601. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.. Courtesy of: Francesco Paonessa.

<p>Immunohistochemistry analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (SMC-601). Tissue: Brain slice. Species: Mouse. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (SMC-601) at 1:500 for Overnight at 4C. Secondary Antibody: Anti-Rabbit IgG: AlexaFluor 488. Counterstain: DAPI at 1:1000 for 5 min. (A) Pons of Non-Tg mouse. (B) Pons of P301SxUBQLN2 Tg mouse. (C) Prefrontal cortex of Non-Tg mouse. (D) Prefrontal cortex of P301SxUBQLN2 Tg mouse. IHC Protocol: 1. Post-fix brains in 4% PFA for 24 hours and put through a 10-30% sucrose gradient. 2. Section by cryostat at 10 uM thickness. 3. Fix in MeOH 15 min. 4. 3×10 min wash in PBS 1X. 5. Heat via microwave in 10mM Citrate Buffer, pH 6 for 4 min at power level 20. 6. Cool in solution for 20 min. 7. Wash 2×5 min in PBS. 8. Permeabilize in 0.5% Triton-X 100 in PBS 10 min. 9. Wash in PBS 10 min. 10. Block for 1 hour in 5% goat serum. 11. Incubate primary Ab (SMC-601 at 1:500) in blocking solution overnight at 4C. 12. Wash 3×10 min in PBS. 13. Incubate in secondary Ab Rb IgG Alexa-fluor 488. 14. Wash 3×10 min in PBS. 15. Incubate in DAPI 1:1000 for 5 min. 16. Wash 3×5 min. 17. Coverslip with Prolong-Gold.. Courtesy of: Julia Gerson, University of Michigan.</p>

Immunohistochemistry analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (SMC-601). Tissue: Brain slice. Species: Mouse. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (SMC-601) at 1:500 for Overnight at 4C. Secondary Antibody: Anti-Rabbit IgG: AlexaFluor 488. Counterstain: DAPI at 1:1000 for 5 min. (A) Pons of Non-Tg mouse. (B) Pons of P301SxUBQLN2 Tg mouse. (C) Prefrontal cortex of Non-Tg mouse. (D) Prefrontal cortex of P301SxUBQLN2 Tg mouse. IHC Protocol: 1. Post-fix brains in 4% PFA for 24 hours and put through a 10-30% sucrose gradient. 2. Section by cryostat at 10 uM thickness. 3. Fix in MeOH 15 min. 4. 3×10 min wash in PBS 1X. 5. Heat via microwave in 10mM Citrate Buffer, pH 6 for 4 min at power level 20. 6. Cool in solution for 20 min. 7. Wash 2×5 min in PBS. 8. Permeabilize in 0.5% Triton-X 100 in PBS 10 min. 9. Wash in PBS 10 min. 10. Block for 1 hour in 5% goat serum. 11. Incubate primary Ab (SMC-601 at 1:500) in blocking solution overnight at 4C. 12. Wash 3×10 min in PBS. 13. Incubate in secondary Ab Rb IgG Alexa-fluor 488. 14. Wash 3×10 min in PBS. 15. Incubate in DAPI 1:1000 for 5 min. 16. Wash 3×5 min. 17. Coverslip with Prolong-Gold.. Courtesy of: Julia Gerson, University of Michigan.

<p>Immunohistochemistry analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (SMC-601). Tissue: Brain slice. Species: Mouse. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (SMC-601) at 1:500 for Overnight at 4C. Secondary Antibody: Anti-Rabbit IgG: AlexaFluor 488. Counterstain: DAPI at 1:1000 for 5 min. CA3 Region of P301SxUBQLN2 Tg mouse. IHC Protocol: 1. Post-fix brains in 4% PFA for 24 hours and put through a 10-30% sucrose gradient. 2. Section by cryostat at 10 uM thickness. 3. Fix in MeOH 15 min. 4. 3×10 min wash in PBS 1X. 5. Heat via microwave in 10mM Citrate Buffer, pH 6 for 4 min at power level 20. 6. Cool in solution for 20 min. 7. Wash 2×5 min in PBS. 8. Permeabilize in 0.5% Triton-X 100 in PBS 10 min. 9. Wash in PBS 10 min. 10. Block for 1 hour in 5% goat serum. 11. Incubate primary Ab (SMC-601 at 1:500) in blocking solution overnight at 4C. 12. Wash 3×10 min in PBS. 13. Incubate in secondary Ab Rb IgG Alexa-fluor 488. 14. Wash 3×10 min in PBS. 15. Incubate in DAPI 1:1000 for 5 min. 16. Wash 3×5 min. 17. Coverslip with Prolong-Gold.. Courtesy of: Julia Gerson, University of Michigan.</p>

Immunohistochemistry analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (SMC-601). Tissue: Brain slice. Species: Mouse. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (SMC-601) at 1:500 for Overnight at 4C. Secondary Antibody: Anti-Rabbit IgG: AlexaFluor 488. Counterstain: DAPI at 1:1000 for 5 min. CA3 Region of P301SxUBQLN2 Tg mouse. IHC Protocol: 1. Post-fix brains in 4% PFA for 24 hours and put through a 10-30% sucrose gradient. 2. Section by cryostat at 10 uM thickness. 3. Fix in MeOH 15 min. 4. 3×10 min wash in PBS 1X. 5. Heat via microwave in 10mM Citrate Buffer, pH 6 for 4 min at power level 20. 6. Cool in solution for 20 min. 7. Wash 2×5 min in PBS. 8. Permeabilize in 0.5% Triton-X 100 in PBS 10 min. 9. Wash in PBS 10 min. 10. Block for 1 hour in 5% goat serum. 11. Incubate primary Ab (SMC-601 at 1:500) in blocking solution overnight at 4C. 12. Wash 3×10 min in PBS. 13. Incubate in secondary Ab Rb IgG Alexa-fluor 488. 14. Wash 3×10 min in PBS. 15. Incubate in DAPI 1:1000 for 5 min. 16. Wash 3×5 min. 17. Coverslip with Prolong-Gold.. Courtesy of: Julia Gerson, University of Michigan.

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