StressXpress®
Corticosterone EIA Kit

Colorimetric detection of corticosterone

Catalog No. SKT-205

5 out of 5 based on 1 customer rating
Species Reactivity ALL
Sample Types EDTA Plasma, Heparin Plasma, Serum, Tissue Culture Media, Urine
SKU: SKT-205 Categories: ,

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SKT-205_Corticosterone_EIA_Kit_Standard_Curve_Fig2
Graph of the Linearity Recovery for the Corticosterone EIA Kit StressXpress® - SKT-205Chemical Structure of the Corticosterone for the Corticosterone EIA Kit StressXpress® - SKT-205
Product Name Corticosterone EIA Kit
Description

Colorimetric detection of corticosterone

Species Reactivity Species Independent
Platform Microplate
Sample Types EDTA Plasma, Heparin Plasma, Serum, Tissue Culture Media, Urine
Detection Method Colorimetric Assay
Assay Type Sandwich EIA (Enzyme Immunoassay)
Utility EIA kit used to measure the corticosterone present in samples.
Sensitivity 24.7 pg/mL
Assay Range 78.125 - 10,000 pg/ml
Precision Intra Assay Precision: Four human samples were diluted with Assay Buffer and run in replicates of 24 in an assay. The mean and precision of the calculated Corticosterone concentrations were: Sample 1- 322.5 pg/mL, 2.6% CV Sample 2- 564.3 pg/mL, 2.8% CV Sample 3- 630.3 pg/mL, 3.1% CV Inter Assay Precision: Three human samples were diluted with Assay Buffer and run in replicates of 24 in two assays. The mean and precision of the calculated Corticosterone concentrations were: Sample 1- 732.6 pg/mL, 1.6% CV Sample 2- 402.7 pg/mL, 0.5% CV Sample 3- 594.5 pg/mL, 0.2% CV
Number of Samples 39 samples in duplicate
Other Resources Kit Booklet Lot No. SC989258 , Kit Booklet Lot No. SC188662 , MSDS , Steroid Solid Extraction Protocol ,
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Temperature 4ºC and -20ºC
Shipping Temperature Blue Ice
Product Type EIA Kits
Assay Overview The Corticosterone EIA kit is designed to quantitatively measure Corticosterone present in serum, plasma, urine, extracted dried fecal samples, and tissue culture media samples. This kit measures total corticosterone in serum and plasma and in extracted fecal samples. A corticosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. We provide protocols on page 8 to prepare assay standards from 5,000 to 78.125 pg/mL or from 10,000 to 78.125 pg/mL. Please choose the standard range that fits your sample concentrations most appropriately. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A corticosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of the corticosterone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.
Kit Overview
Component No.ItemQuantity / Size
SKC-205A Coated Clear 96 Well Plates 1 Each
SKC-205B Corticosterone Standard 125 µL
SKC-205C StressXpress® Corticosterone Antibody 3 mL
SKC-205D StressXpress® Corticosterone: HRP Concentrate 50 µL
SKC-205E StressXpress® Corticosterone: HRP Diluent 5 mL
SKC-205F Assay Buffer 50 mL
SKC-205G Dissociation Reagent 1 mL
SKC-205H Wash Buffer Concentrate 50 mL
SKC-205I TMP Substrate 11 mL
SKC-205J Stop Solution 5 mL
SKT-205K Plate Sealer 1 Each
Cite This Product Corticosterone EIA Kit (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SKT-205)

Biological Description

Alternative Names (11β)-​11,​21-​dihydroxypregn-​4-​ene-​3,​20-​dione EIA Kit
Research Areas Cancer, Cell Signaling, Neuroscience, Oxidative Stress
Scientific Background Corticosterone (C21H30O4, Kendall’s Compound ‘B’) is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to stimulation of the adrenal cortex by ACTH and is the precursor of aldosterone. Corticosterone is a major indicator of stress and is the major stress steroid produced in non-human mammals. Studies involving corticosterone and levels of stress include impairment of long term memory retrieval (1), chronic corticosterone elevation due to dietary restrictions (2) and in response to burn injuries (3). In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns (4,5).
References 1. Hupé, JM, et al. Nature, 1998; 394: 784-787.
2. Kitaysky AS, Kitaiskaia EV, Wingfield JC, Piatt JF. J. Comp. Physiol, 2001; 171: 701-709.
3. Thellin O, Noel G, Khuana S, Ogle CK and Horseman ND. Shock, 2001; 16(5): 393-397.
4. Krame, KM. and Sothern RB. Chronobiol. Int., 2001; 18(6): 933-945.
5. Vazquez-Palacios G, et al. Pharmacol. Biochem Behavior, 2001: 70(2-3): 305-310.

Product Images

<p>Chemical structure of Corticosterone</p>

Chemical structure of Corticosterone

<p>Typical Standard Curve for the Corticosterone EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-205. Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: 78.125 – 10,000 pg/ml.</p>

Typical Standard Curve for the Corticosterone EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-205. Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: 78.125 – 10,000 pg/ml.

<p>Linearity was determined by taking two serum samples treated with Dissociation Reagent and diluted 1:50 with Assay Buffer, one with a low diluted corticosterone level of 104.6 pg/mL and one with a higher diluted level of 2,890.5 pg/mL, and mixing them in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used.</p>

Linearity was determined by taking two serum samples treated with Dissociation Reagent and diluted 1:50 with Assay Buffer, one with a low diluted corticosterone level of 104.6 pg/mL and one with a higher diluted level of 2,890.5 pg/mL, and mixing them in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used.

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