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| Product Name | Creatinine Serum Detection Kit |
| Description |
Colorimetric measurement of creatinine |
| Species Reactivity | Human, Mouse, Rabbit, Rat, Sheep |
| Platform | Microplate |
| Sample Types | Plasma, Serum |
| Detection Method | Colorimetric Assay |
| Assay Type | Direct Quantitative Assay |
| Utility | Colorimetric assay used to measure creatinine in samples. |
| Sensitivity | 0.085 mg/dL |
| Assay Range | 0.5 - 4 mg/dl |
| Precision | Intra Assay Precision: Four human serum samples were run in triplicate on a same-day assay determined that the mean and precision of the calculated concentrations were: Sample 1- 0.75 mg/dL, 5% CV Sample 2- 0.78 mg/dL, 6% CV Sample 3- 0.6 mg/dL, 4% CV Sample 4- 0.76 mg/dL, 6% CV Inter Assay Precision: Four human serum samples were run in triplicate on a same-day assay determined that the total range of 4-6% represents the variation between different assays. |
| Number of Samples | 91 samples in duplicate |
| Other Resources | Kit Booklet , Kit Booklet Lot No. SC188754 , MSDS , Creatinine Serum Kit Excel Analysis Worksheet |
| Field of Use | Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only. |
Properties
| Storage Temperature | 4ºC | |||||||||||||||
| Shipping Temperature | Blue Ice | |||||||||||||||
| Product Type | Detection Kits | |||||||||||||||
| Assay Overview | The Creatinine Serum Detection Kit is designed to quantitatively measure creatinine present in serum samples. A creatinine standard, calibrated to a creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or samples are pipetted into a clear microtiter plate. An assay diluent is added to all standards, controls and samples. The color generating reaction is initiated with the StressXpress® Creatinine Reagent, which is pipetted into each well. The assay utilizes a kinetic absorbance method to overcome interference by colored compounds in serum. The absorbance of the colored product is read in a microtiter plate reader capable of measuring 490nm wavelength. For a total of 30 minutes, the optical density is read at 1 minute intervals. The concentration of creatinine is calculated using the Kinetic Mode software and samples are compared to the curve generated from the standards, or by using the Excel worksheet available for free download at our web site. The Jaffe reaction used in this kit has been modified to read creatinine levels in serum (8). | |||||||||||||||
| Kit Overview |
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| Cite This Product | Creatinine Serum Detection Kit (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SKT-217) |
Biological Description
| Alternative Names | N-Carbamimidoyl-N-methylglycine Detection Kit, Methylguanidoacetic acid Detection Kit |
| Research Areas | Cardiovascular System, Cell Signaling |
| Scientific Background | Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP (1). Creatine either comes from the diet or synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist (2). Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo , this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine (3). Under normal conditions, its formation occurs at a rate that is relatively constant and as intra- individual variation is <15% from day to day, creatinine is a useful tool for normalizing the levels of other molecules found in urine. Additionally altered creatinine levels may be associated with other conditions that result in decreased renal blood flow such as diabetes and cardiovascular disease (4-6). |
| References |
1. Wallimann, T. et al., Biochem. J., 2000, 281, 21-40. 2. Wyss, M. and Kaddurah-Daouk, R., Physiol. Rev., 2000, 80, 1107-1213. 3. Raja Iyengar, M. et al., J. Biol. Chem, 1985, 260, 7562-7567. 4. Manjunath, G. et al., Postgrad. Med. 2001, 110, 55-62. 5. Gross, J.L. et al., Diabetes Care, 2005, 28, 164-176. 6. Anavekar, N.S. et al., New Engl. J. Med., 2004, 351, 1285-1295. |
Product Images
Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine.
Typical Standard Curve for the Creatinine Serum Detection Kit () StressXpress® – SKT-217. Assay Type: Direct Enzyme. Detection Method: Colorimetric Assay. Assay Range: 0.5 – 4 mg/dl.
Linearity was determined by taking two human serum samples, one with a low diluted creatinine level of 0.58 mg/dL and one with a higher level of 1.23 mg/dL and mixing them in given ratios. The measured concentrations were compared to the expected values.
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Based on validation through cited publications.