StressXpress®
HO-1 ELISA Kit

Colorimetric detection of heme oxygenase 1

Catalog No. SKT-111

5 out of 5 based on 1 customer rating
Species Reactivity Hu, Rt
Sample Types Cell lysates, Cyst fluid, Serum, Tissue
SKU: SKT-111 Categories: ,

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SKT-111_HO-1_ELISA_Kit_Standard_Curve.png
Product Name HO-1 ELISA Kit
Description

Colorimetric detection of heme oxygenase 1

Species Reactivity Human, Rat
Platform Microplate
Sample Types Cell lysates, Cyst fluid, Serum, Tissue
Detection Method Colorimetric Assay
Assay Type Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
Utility ELISA kit used to quantitate HO-1 concentration in samples.
Sensitivity 0.21 ng/ml
Assay Range 0.781 - 50 ng/mL
Incubation Time 30 minutes
Number of Samples 40 samples in duplicate
Other Resources Kit Booklet Lot No. SH334100 , Kit Booklet Lot No. SH111999 , Kit Booklet Lot No. SH288446 , Kit Booklet , MSDS
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Temperature 4ºC and -20ºC
Shipping Temperature Blue Ice
Product Type ELISA Kits
Assay Overview 1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 50 µL of Pre-Treatment Buffer to all sample and standard wells. 3. Add 50 µL of Standard and sample to appropriate wells. 4. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 2 hours. 5. Wash plate four times with 1X Wash Buffer. 6. Add 100 µL of Biotinylated Antibody Working Solution to each well. 7. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 8. Wash plate four times with 1X Wash Buffer as described in step 5. 9. Add 100 µL of Streptavidin-HRP Working Solution to each well. 10. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 11. Wash plate four times with 1X Wash Buffer as described in step 5. 12. Add 100 µL of TMB Substrate to each well. 13. Develop the plate in the dark at room temperature for 30 minutes. 14. Stop reaction by adding 100 µL of Stop Solution to each well. 15. Measure absorbance on a plate reader at 450 nm.
Kit Overview
Component No.ItemQuantity / Size
SKC-111A Anti-HO-1 Immunoassay Plate 1 Plate
SKC-111B 5X HO-1 Extraction Reagent 1 vial/10 ml
SKC-111C Recombinant HO-1 Standard 2 vials
SKC-111D Standard and Sample Diluent 1 vial/ 50 ml
SKC-111E 10X Wash Buffer Concentrate 1 vial/100 ml
SKC-111F Anti-HO-1 Biotinylated Antibody Concentrate 1 vial/150 µl
SKC-111G Anti-HO-1 Biotinylated Antibody Diluent 1 vial/ 13 ml
SKC-111H Streptavidin: HRP Concentrate 1 vial/150 µl
SKC-111I Streptavidin: HRP Diluent 1 vial/ 13 ml
SKC-111J TMB Substrate 1 vial/ 13 ml
SKC-111K Stop Solution 1 vial/ 13 ml
SKC-111L Pre-treatment Buffer 1 vial/ 13 ml
Cite This Product HO-1 ELISA Kit (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SKT-111)

Biological Description

Alternative Names Heme oxygenase 1 ELISA Kit, Hemox ELISA Kit, HMOX1 ELISA Kit, HO1 ELISA Kit, HO 1 ELISA Kit, HSP32 ELISA Kit
Research Areas Cancer, Oxidative Stress
Scientific Background Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
References 1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86.
2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47.
3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419.
4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8.
5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925–10930.
6. Yet S.F., et al. (2003) FASEB J. 17: 1759–1761.
7. Yachie A., et al. (1999) J Clin Invest. 103: 129–135.

Product Images

<p>Typical Standard Curve for the HO-1 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-111. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.781 – 50 ng/mL.</p>

Typical Standard Curve for the HO-1 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-111. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.781 – 50 ng/mL.

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