MOLECULAR SIGNATURE®
VPS35 Kit

Colorimetric measurement of VPS35

Catalog No. SKT-141

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Species Reactivity Human, Mouse, Rat
Sample Types Cell Lysate, Tissue
SKU: SKT-141 Categories: ,

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SKT-141_VPS35_Kit_Typical_Standard_Curve
Graph of the Linearity Recovery for the VPS35 Kit - SKT-141Graph of the VPS35 Determination of WT-A549 vs KN-549 Lysates Graph for the VPS35 Kit MOLECULAR SIGNATURE® - SKT-141
Product Name VPS35 Kit
Description

Colorimetric measurement of VPS35
Species Reactivity Human, Mouse, Rat
Platform Microplate
Sample Types Cell Lysate, Tissue
Detection Method Colorimetric
Assay Type Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
Sensitivity 50 pg/mL
Assay Range 78.125 - 5000 pg/mL
Incubation Time 3 hours
Number of Samples 40 samples in duplicate
Other Resources Kit Booklet Lot No. SV888883 , Kit Booklet Lot No. SV999550 , Kit Booklet Lot No. SV888770 , MSDS
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Temperature 4ºC and -20ºC
Shipping Temperature Blue Ice
Product Type ELISA Kits
Kit Overview
Component No.ItemQuantity / Size
SKC-141A Anti-VPS35 Immunoassay Plate 1 Plate
SKC-141B 5X VPS35 Extraction Reagent 1 bottle/10mL
SKC-141C Recombinant VPS35 Standard 2 vials
SKC-141D Standard and Sample Diluent (Red) 1 bottle/50mL
SKC-141E 10X Wash Buffer Concentrate 1 bottle/100mL
SKC-141F Anti-VPS35 Biotinylated Antibody Concentrate 1 vial/150uL
SKC-141G Anti-VPS35 Biotinylated Antibody Diluent (Green) 1 bottle/13mL
SKC-141H 100X Streptavidin:HRP Concentrate 1 vial/150uL
SKC-141I Streptavidin: HRP Diluent (Purple) 1 bottle/13mL
SKC-141J TMB Substrate 1 bottle/13mL
SKC-141K Stop Solution 1 bottle/13mL

Biological Description

Research Areas Alzheimer's Disease, Cell Signaling, Golgi Proteins, Membrane Trafficking Proteins, Neurodegeneration, Neuroscience, Parkinson's Disease, Protein Trafficking
Scientific Background Vacuolar Protein Sorter-35 (VPS35) is a component of the retromer complex, which is essential for endosome-to-Golgi retrieval of membrane proteins. VPS35 mutations such as D620N have been linked to Parkinson’s Disease (PD) (1,2) and affect retromer function, protein homeostasis, and mitochondria (3).
References 1. Vilarino-Guell, C. et al. (2011) Am J Hum Genet 89:162–167
2. Zimprich, A. et al. (2011) Am J Hum Genet 89:168–175
3. Rahman, A.A., Morrison, B.E. (2019) Neurosci 401:1-10.

Product Images

<p>Typical Standard Curve for the VPS35 Kit (Enzyme-Linked Immunosorbent Assay) MOLECULAR SIGNATURE® – SKT-141. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay.</p>

Typical Standard Curve for the VPS35 Kit (Enzyme-Linked Immunosorbent Assay) MOLECULAR SIGNATURE® – SKT-141. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay.

<p>VPS35 Determination of WT-A549 vs KN-549 Lysates Graph for the VPS35 Kit (Enzyme-Linked Immunosorbent Assay) MOLECULAR SIGNATURE® – SKT-141. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay.</p>

VPS35 Determination of WT-A549 vs KN-549 Lysates Graph for the VPS35 Kit (Enzyme-Linked Immunosorbent Assay) MOLECULAR SIGNATURE® – SKT-141. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay.

<p>Linearity was determined by using low (rat kidney) and high (mouse kidney) natural samples, diluted 1/100 and mixed in various proportions.</p>

Linearity was determined by using low (rat kidney) and high (mouse kidney) natural samples, diluted 1/100 and mixed in various proportions.

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