Product Name | Biotinyl Tyramide |
Description |
Signal Amplifier |
Purity | >98% (TLC); NMR (Conforms) |
CAS No. | 41994-02-9 |
Molecular Formula | C18H25N3O3S |
Molecular Weight | 363.5 |
Field of Use | Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only. |
Storage Temperature | -20ºC |
Shipping Temperature | Shipped Ambient |
Product Type | Amplifier |
Solubility | May be dissolved in DMSO (35 mg/ml); or ethanol (20 mg/ml) |
Source | Synthetic |
Appearance | White powder |
SMILES | [H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCC3=CC=C(C=C3)O)=O |
InChI | InChI=1S/C18H25N3O3S/c22-13-7-5-12(6-8-13)9-10-19-16(23)4-2-1-3-15-17-14(11-25-15)20-18(24)21-17/h5-8,14-15,17,22H,1-4,9-11H2,(H,19,23)(H2,20,21,24)/t14-,15-,17-/m0/s1 |
InChIKey | VZWXNOBHWODXCW-ZOBUZTSGSA-N |
Safety Phrases |
Classification: Not a hazardous substance or mixture. Safety Phrases: S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. |
Cite This Product | Biotinyl Tyramide (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-573) |
Alternative Names | (3aS,4S,6aR)-Hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide; Biotin-phenol |
Research Areas | Cell Signaling, Neuroscience |
PubChem ID | 21826423 |
Scientific Background | Biotinyl Tyramide is a biotin derivative that can be used as a reagent to amplify both IHC signals and in situ hybridization protocols (1). The secondary should be an HRP-labelled antibody or streptavidin conjugate; catalysis by the HRP results in the deposition of multiple tyramide molecules which can then be detected with a labeled streptavidin conjugate. Sensitivity has found to be 100 fold. It can be used with both chromogenic and fluorescent visualization methods (2). |
References | 1. Bobrow M.N. et al., (1989) J. Immunol. Methods. 125:279. 2. Kim S.H., et al. (2003) J Histochem Cytochem. 51(1): 129-132. |
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